Zhang Xiao-Gang, Wang Yan-Hua, Han Cong, Hu Shan, Wang Li-Qiang, Hu Jian-Hong
College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
Cryobiology. 2015 Jun;70(3):246-52. doi: 10.1016/j.cryobiol.2015.03.004. Epub 2015 Mar 25.
Trehalose is widely used for cryopreservation of various cells and tissues. Until now, the effect of trehalose supplementation on cell viability and antioxidant enzyme activity in frozen-thawed bovine calf testicular tissue remains unexplored. The objective of the present study was to compare the effect of varying doses of trehalose in cryomedia on cell viability and key antioxidant enzymes activities in frozen-thawed bovine calf testicular tissue. Bovine calf testicular tissue samples were collected and cryopreserved in the cryomedias containing varying doses (0, 5, 10, 15, 20 and 25%; v/v) of trehalose, respectively. Cell viability, total antioxidant capacity (T-AOC) activity, catalase (CAT) activity, superoxide dismutase (SOD) activity, glutathione (GSH) content and malondialdehyde (MDA) content were measured and analyzed. The results showed that cell viability, T-AOC activity, SOD activity, CAT activity and GSH content of frozen-thawed bovine calf testicular tissue was decreased compared with that of fresh group (P<0.05). MDA content in frozen-thawed bovine calf testicular tissue was significantly increased compared with that of fresh group (P<0.05). The cryomedia added 15% trehalose exhibited the greatest percentage of cell viability and antioxidant enzyme activity (SOD and CAT) among frozen-thawed groups (P<0.05). Meanwhile, GSH content was the lowest among frozen-thawed groups (P<0.05). However, there were no significance differences in MDA content among the groups added 10, 15 and 20% trehalose (P>0.05). In conclusion, the cryomedia added 15% trehalose reduced the oxidative stress and improved the cryoprotective effect of bovine calf testicular tissue. Further studies are required to obtain more concrete results on the determination of antioxidant capacity of trehalose in frozen-thawed bovine calf testicular tissue.
海藻糖被广泛用于各种细胞和组织的冷冻保存。到目前为止，海藻糖补充剂对冻融后的牛犊睾丸组织中细胞活力和抗氧化酶活性的影响尚未得到研究。本研究的目的是比较不同剂量海藻糖添加到冷冻保护剂中对冻融后的牛犊睾丸组织中细胞活力和关键抗氧化酶活性的影响。收集牛犊睾丸组织样本，分别保存在含有不同剂量（0、5、10、15、20和25%；v/v）海藻糖的冷冻保护剂中。测量并分析细胞活力、总抗氧化能力（T-AOC）活性、过氧化氢酶（CAT）活性、超氧化物歧化酶（SOD）活性、谷胱甘肽（GSH）含量和丙二醛（MDA）含量。结果表明，与新鲜组相比，冻融后的牛犊睾丸组织的细胞活力、T-AOC活性、SOD活性、CAT活性和GSH含量均降低（P<0.05）。与新鲜组相比，冻融后的牛犊睾丸组织中MDA含量显著增加（P<0.05）。在冻融组中，添加15%海藻糖的冷冻保护剂显示出最高的细胞活力百分比和抗氧化酶活性（SOD和CAT）（P<0.05）。同时，GSH含量在冻融组中最低（P<0.05）。然而，添加10%、15%和20%海藻糖的组之间MDA含量没有显著差异（P>0.05）。总之，添加15%海藻糖的冷冻保护剂降低了氧化应激并提高了牛犊睾丸组织的冷冻保护效果。需要进一步研究以获得关于冻融后的牛犊睾丸组织中海藻糖抗氧化能力测定的更具体结果。
