Rosenthal A, Mesrobian A
Invest Radiol. 1975 Mar-Apr;10(2):140-47. doi: 10.1097/00004424-197503000-00007.
The acute effects of an injection of contrast material (Renovist) on intraerythrocytic and extracellular pH was studied at cardiac catheterization in 72 patients with congenital heart disease. A decrease in mean extracellular (plasma) pH (7.444 plus or minus .006 to 7.419 plus or minus 0.009) and an increase in mean intraerythrocytic pH (7.204 plus or minus .005 to 7.232 plus or minus .006) were observed within minutes after injection (p smaller than .01). In 17/72 patients, simultaneous measurements of oxygen affinity for hemoglobin as characterized by P50 (oxygen tension at 50% O-2 saturation) corrected to in vivo arterial pH decreased from a mean of 26.4 to 25.2 mm Hg (p smaller than .01). It is postulated that the acute increase in intraerythrocyte pH and increased affinity of hemoglobin for oxygen are due to a decrease in intracellular hydrogen ion concentration induced by the increase in plasma osmolality with subsequent shift of hemoglobin oxygen equilibrium via the Bohr effect.
在72例先天性心脏病患者的心导管检查中，研究了注射造影剂（Renovist）对红细胞内和细胞外pH值的急性影响。注射后数分钟内观察到平均细胞外（血浆）pH值降低（从7.444±0.006降至7.419±0.009），平均红细胞内pH值升高（从7.204±0.005升至7.232±0.006）（p<0.01）。在72例患者中的17例中，同时测量的以P50（50%O₂饱和度时的氧分压）为特征的血红蛋白氧亲和力，校正至体内动脉pH值后，从平均26.4降至25.2mmHg（p<0.01）。据推测，红细胞内pH值的急性升高和血红蛋白对氧亲和力的增加是由于血浆渗透压升高导致细胞内氢离子浓度降低，随后通过玻尔效应使血红蛋白氧平衡发生移动所致。
