Miller A G, Budd K
J Bacteriol. 1975 Jan;121(1):91-8. doi: 10.1128/jb.121.1.91-98.1975.
The uptake of Cl minus by the ascomycetes Neocosmospora vasinfecta was investigated. Intramycelial concentrations of more than 55 mM (890-fold the external concentration) were reached. Accumulation was as inorganic Cl minus and nystatin induced total loss of mycelial Cl minus without extensive protein loss, implying that Cl minus retention was not due to binding to macromolecules. Cl minus transport was largely unidirectional with efflux being low under all conditions. Uptake was temperature dependent (maximal Arrhenius activation energy of 18.0 kcal/mol) and was severely reduced by KCN, dicyclohexylcarbodiimide, and anaerobiosis. A comparison with the inhibition of oxygen uptake under the same conditions implied that Cl minus transport was not directly coupled to aerobic electron transport. Cl minus uptake was a saturatable function of the external Cl minus concentration, and apparent Km values of 6.4 times 10-6 M and 10-4 M were calculated. Of the anions tested, only Br minus effectively inhibited Cl minus uptake and I minus, NO3 minus, SO4 minus 2, HCO3 minus, and H2PO4 minus were without effect. Cl minus uptake did not require concomitant cation uptake.
对子囊菌新暗色孢菌吸收氯离子的情况进行了研究。菌丝体内氯离子浓度超过55毫摩尔（是外部浓度的890倍）。积累的是无机氯离子，制霉菌素会导致菌丝体氯离子完全丧失，且蛋白质损失不大，这意味着氯离子的保留并非由于与大分子结合。氯离子运输在很大程度上是单向的，在所有条件下外流都很低。吸收依赖于温度（最大阿累尼乌斯活化能为18.0千卡/摩尔），并被氰化钾、二环己基碳二亚胺和厌氧条件严重抑制。与相同条件下对氧气吸收的抑制作用进行比较表明，氯离子运输并非直接与有氧电子传递相偶联。氯离子吸收是外部氯离子浓度的饱和函数，计算得出的表观米氏常数分别为6.4×10⁻⁶摩尔和10⁻⁴摩尔。在所测试的阴离子中，只有溴离子能有效抑制氯离子吸收，而碘离子、硝酸根离子、硫酸根离子、碳酸氢根离子和磷酸二氢根离子则没有影响。氯离子吸收不需要伴随阳离子吸收。
