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大肠杆菌中的阳离子转运。II. 细胞内氯离子浓度。

Cation transport in Escherichia coli. II. Intracellular chloride concentration.

作者信息

SCHULTZ S G, WILSON N L, EPSTEIN W

出版信息

J Gen Physiol. 1962 Sep;46(1):159-66. doi: 10.1085/jgp.46.1.159.

Abstract

The intracellular Cl concentration in E. coli has been studied as a function of the Cl concentration in the growth medium and the age of the bacterial culture. The ratio of extracellular to intracellular Cl concentration is shown to be 3.0 in the logarithmic phase and 1.13 in the stationary phase, both ratios being independent of the extracellular Cl concentration. If it may be assumed that Cl is passively distributed in this organism, these results are consistent with a transmembrane P.D. of 29 mv, interior negative, during the logarithmic phase, and 3 mv, interior negative, in the stationary phase.

摘要

人们研究了大肠杆菌细胞内氯离子浓度与生长培养基中氯离子浓度以及细菌培养物菌龄之间的关系。结果表明,在对数生长期,细胞外与细胞内氯离子浓度之比为3.0,而在稳定期该比值为1.13,这两个比值均与细胞外氯离子浓度无关。如果可以假定氯离子在这种生物体中是被动分布的,那么这些结果与对数生长期跨膜电位为29毫伏(细胞内为负)以及稳定期跨膜电位为3毫伏(细胞内为负)是一致的。

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