Ding M, Zeng J, Sroussi H, Yu J, Xu J, Cheng X, Fan Y
Department of Anatomy and cell biology, Rush University Medical Center, Chicago, IL, USA.
Oral Dis. 2014 Mar;20(2):205-11. doi: 10.1111/odi.12090. Epub 2013 Mar 18.
Oral lichen planus (OLP) is a prevalent cell-mediated autoimmune disease of unknown etiology. Disruption in the Th1/Th2 equilibrium has been suspected to contribute to the pathogenesis of OLP. In this study, we aimed at exploring, in OLP, the interactions between Th1/Th2 cytokines and Golli-MBP, a regulator of autogenic T cells.
Thirty-six OLP patients aged 18-79 and nineteen control subjects aged 20-69 were enrolled in the dental clinics of Nanjing Medical University. OLP was diagnosed clinically and verified by histopathological examination. All subjects were free of other autoimmune diseases. Peripheral blood mononuclear cells (PBMC) and serum were collected from all subjects. Gene expression and protein levels of Golli-MBP, IFN-γ and IL-4 were measured, respectively, by semiquantitative reverse transcription polymerase chain reaction and enzyme-linked immunosorbent assay.
When compared with control, PBMC and serum from OLP patients exhibited a significantly higher expression and concentration of Golli-MBP and IL-4 and lower levels of IFN-γ. A strong negative correlation (r = -0.838, P < 0.000) was detected between the ratio of IFN-γ/IL-4 and Golli-MBP gene expression in PBMCs of OLP patients.
These data support a potential link between Golli-MBP and the dysregulation of the Th1/Th2 equilibrium in OLP.
口腔扁平苔藓（OLP）是一种病因不明的常见细胞介导的自身免疫性疾病。Th1/Th2平衡的破坏被怀疑与OLP的发病机制有关。在本研究中，我们旨在探讨OLP中Th1/Th2细胞因子与自体T细胞调节剂Golli-MBP之间的相互作用。
南京医科大学牙科诊所招募了36名年龄在18 - 79岁的OLP患者和19名年龄在20 - 69岁的对照受试者。OLP通过临床诊断并经组织病理学检查证实。所有受试者均无其他自身免疫性疾病。从所有受试者中采集外周血单个核细胞（PBMC）和血清。分别通过半定量逆转录聚合酶链反应和酶联免疫吸附测定法测量Golli-MBP、IFN-γ和IL-4的基因表达和蛋白水平。
与对照组相比，OLP患者的PBMC和血清中Golli-MBP和IL-4的表达和浓度显著更高，而IFN-γ水平更低。在OLP患者PBMC中，IFN-γ/IL-4比值与Golli-MBP基因表达之间检测到强负相关（r = -0.838，P < 0.000）。
这些数据支持Golli-MBP与OLP中Th1/Th2平衡失调之间的潜在联系。
