Nimrod A, Ryan K J
J Clin Endocrinol Metab. 1975 Mar;40(3):367-72. doi: 10.1210/jcem-40-3-367.
The ability of human abdominal, breast and axillary fat to convert androgens into estrogens was investigated by incubating labeled substrates in the presence of NADPH with a variety of cell preparations. The incubation products were subjected to phenolic partition, paper chromatography, methyl-ether formation, repeat chromatography and crystallization with cold carrier reference standards to constant specific activity. Androstenedione was converted to estrone and, to a lesser extent, to 17beta-estradiol by crude homogenates, minces, fat-free particulate fractions (1,000-100,000 time g) and isolated fat cells obtained from abdominal, breast or axillary fat. Testosterone was found to be aromatized as actively as androstenedione, but inthis case more 17 beta-estrodiol was formed than estrone. 19-Hydroxyandrostenedione-2 also served as substrate, givingresults similar to those obtained with androstenedione. Fat tissue obtained from cancerous breasts was found to be as active as normal breast fat (1-4 pg/g fat/90 min) and within the range found for abdominal fat (1-27 pg/g fat/90 min). In each case in which axillary fat was compared to breast fat from the same subject, the activity of the axillary fat was 5 to 10 times higher. The results indicate a possible role of adipose tissue as a significant extra-gonadal source of estrogens.
通过在NADPH存在的情况下，将标记底物与各种细胞制剂一起孵育，研究了人体腹部、乳房和腋窝脂肪将雄激素转化为雌激素的能力。将孵育产物进行酚类分配、纸色谱、甲基醚形成、重复色谱，并与冷载体参考标准品结晶，直至比活性恒定。雄烯二酮可被腹部、乳房或腋窝脂肪的粗匀浆、切碎物、无脂肪颗粒部分（1000 - 100000倍重力）和分离的脂肪细胞转化为雌酮，在较小程度上转化为17β - 雌二醇。发现睾酮与雄烯二酮一样容易发生芳香化作用，但在这种情况下，形成的17β - 雌二醇比雌酮更多。19 - 羟基雄烯二酮 - 2也可作为底物，其结果与用雄烯二酮得到的结果相似。发现从患癌乳房获取的脂肪组织与正常乳房脂肪（1 - 4 pg/g脂肪/90分钟）活性相同，且在腹部脂肪的活性范围内（1 - 27 pg/g脂肪/90分钟）。在每一个将同一受试者的腋窝脂肪与乳房脂肪进行比较的案例中，腋窝脂肪的活性高出5至10倍。结果表明脂肪组织可能作为雌激素的一个重要性腺外来源发挥作用。
