Nagata Y, Nakamura R M, Osborn C E, Naftolin F, Mishell D R
J Lab Clin Med. 1975 Mar;85(3):515-22.
Freezing and thawing of human serum produces alkaline changes in the pH. Weak buffers were of insufficient capacity to compensate for these pH changes. Thus the effect of serum pH on modified solid-phase and double-antibody radioimmunoassay (RIA) of LH and FSH was studied. Pooled postmenopausal serum standardized against the Second International Reference Preparation of Human Menopausal Urinary Gonadotropon was used as a laboratory standard. In the solid phase RIA for LH and FSH, slopes with the greatest maximum binding were obtained at pH 6.4, whereas for the double-antibody method best results were obtained at pH 8.0. Since there is individual variation in the pH of various serum samples, different values will be obtained from samples containing the same amount of hormone. It is sum samples be standardized by the use of buffers with the capacity to keep the pH of the serum constant and that serum standards be dialyzed.
人血清的冻融会导致pH值发生碱性变化。弱缓冲液补偿这些pH值变化的能力不足。因此，研究了血清pH值对促黄体生成素（LH）和促卵泡生成素（FSH）改良固相和双抗体放射免疫测定（RIA）的影响。以第二次国际人绝经后尿促性腺激素参考制剂为标准的绝经后混合血清用作实验室标准品。在LH和FSH的固相RIA中，在pH 6.4时获得最大结合率的斜率，而对于双抗体法，在pH 8.0时获得最佳结果。由于不同血清样本的pH值存在个体差异，因此从含有相同量激素的样本中会获得不同的值。总结而言，样本应使用具有保持血清pH值恒定能力的缓冲液进行标准化，并且血清标准品应进行透析。
