Hans Nidhi, Singh Shailja, Jain S K, Chauhan Virander S
Malaria Group, International Centre for Genetic Engineering and Biotechnology, P. O. Box 10504, Aruna Asaf Ali Marg, New Delhi 110067, India.
Mol Biochem Parasitol. 2013 Mar;188(1):34-9. doi: 10.1016/j.molbiopara.2013.02.007. Epub 2013 Mar 13.
The clinical symptoms of malaria are attributed to the blood stage life cycle of parasite in which merozoite invades erythrocyte, undergoes multiplication and exit to re-invade into new erythrocyte to continue its life cycle. The interaction of repertoire of parasite proteins with host cell receptors is essential for invasion process. Identification, characterization and localization of the proteins involved in invasion will enrich our understanding of this complex process. In the present study we have identified a novel Apical Rhoptry Neck Protein in Plasmodium falciparum, which harbours a predicted signal and transmembrane domain and is conserved across the species. The transcription and translation analysis confirmed its expression in schizont stage of asexual cycle of P. falciparum. Immunoflouresence microscopy in schizonts and merozoites revealed its localization in the neck of rhoptries of P. falciparum. Furthermore, PfARNP has been found at the tight junction during invasion of P. falciparum merozoite to erythrocyte.
疟疾的临床症状归因于疟原虫的血液阶段生命周期，在此过程中，裂殖子侵入红细胞，进行增殖，然后逸出并重新侵入新的红细胞以继续其生命周期。疟原虫蛋白质库与宿主细胞受体的相互作用对于侵入过程至关重要。对参与侵入的蛋白质进行鉴定、表征和定位将丰富我们对这一复杂过程的理解。在本研究中，我们在恶性疟原虫中鉴定出一种新型顶端棒状体颈部蛋白，它具有预测的信号和跨膜结构域，并且在整个物种中保守。转录和翻译分析证实了它在恶性疟原虫无性周期裂殖体阶段的表达。对裂殖体和裂殖子进行免疫荧光显微镜检查显示，它定位于恶性疟原虫棒状体的颈部。此外，在恶性疟原虫裂殖子侵入红细胞的过程中，在紧密连接处发现了PfARNP。
