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热应激条件下印度热带驯化牛和水牛外周血单个核细胞中稳定参考基因的选择。

Selection of stable reference genes in heat stressed peripheral blood mononuclear cells of tropically adapted Indian cattle and buffaloes.

机构信息

National Bureau of Animal Genetic Resources, Karnal, Haryana 132001, India.

出版信息

Mol Cell Probes. 2013 Jun-Aug;27(3-4):140-4. doi: 10.1016/j.mcp.2013.02.003. Epub 2013 Mar 14.

DOI:10.1016/j.mcp.2013.02.003
PMID:23499765
Abstract

Eleven reference genes from different functional categories were assessed for their stable expression pattern in heat stressed PBMCs across Indian buffalo and cattle using GeNorm, NormFinder and BestKeeper algorithms. As the first report, we suggest B2M, RPS9 and RPS15a as suitable reference genes for accurate normalization of PBMC transcript data while the ACTB gene is not recommended.

摘要

使用 GeNorm、NormFinder 和 BestKeeper 算法评估了来自不同功能类别的 11 个参考基因在印度水牛和牛的热应激 PBMC 中的稳定表达模式。作为第一项报告，我们建议 B2M、RPS9 和 RPS15a 作为准确归一化 PBMC 转录数据的合适参考基因，而不建议使用 ACTB 基因。

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