Department of Bioengineering, University of Utah, Salt Lake City, UT 84112, USA.
Acta Biomater. 2013 Jul;9(7):7200-8. doi: 10.1016/j.actbio.2013.02.047. Epub 2013 Mar 14.
Surface-adsorbed fibrinogen (FBG) was recognized by adhering astrocytes, and was removed from the substrates in vitro by a two-phase removal process. The cells removed adsorbed FBG from binary proteins' surface patterns (FBG+laminin, or FBG+albumin) while leaving the other protein behind. Astrocytes preferentially expressed chondroitin sulfate proteoglycan (CSPG) at the loci of fibrinogen stimuli; however, no differences in overall CSPG production as a function of FBG surface coverage were identified. Removal of FBG by astrocytes was also found to be independent of transforming growth factor type β (TGF-β) receptor based signaling as cells maintained CSPG production in the presence of TGF-β receptor kinase inhibitor, SB 431542. The inhibitor decreased CSPG expression, but did not abolish it entirely. Because blood contact and subsequent FBG adsorption are unavoidable in neural implantations, the results indicate that implant-adsorbed FBG may contribute to reactive astrogliosis around the implant as astrocytes specifically recognize adsorbed FBG.
表面吸附的纤维蛋白原 (FBG) 被黏附的星形胶质细胞识别，并通过两相去除过程从体外的基质中去除。细胞从二元蛋白表面模式（FBG+层粘连蛋白或 FBG+白蛋白）中去除吸附的 FBG，而将另一种蛋白质留在后面。星形胶质细胞在纤维蛋白原刺激的部位优先表达硫酸软骨素蛋白聚糖 (CSPG)；然而，在纤维蛋白原表面覆盖率的功能方面，CSPG 的整体产生没有差异。星形胶质细胞对 FBG 的去除也被发现与转化生长因子-β (TGF-β) 受体信号无关，因为在 TGF-β 受体激酶抑制剂 SB 431542 的存在下，细胞维持 CSPG 的产生。抑制剂降低了 CSPG 的表达，但并没有完全消除它。由于在神经植入物中不可避免地会发生血液接触和随后的 FBG 吸附，因此这些结果表明，植入物吸附的 FBG 可能会导致植入物周围的反应性星形胶质细胞增生，因为星形胶质细胞特异性识别吸附的 FBG。
