星形胶质细胞特异性清除表面吸附的纤维蛋白原，并局部表达硫酸软骨素蛋白聚糖。
Astrocytes specifically remove surface-adsorbed fibrinogen and locally express chondroitin sulfate proteoglycans.
机构信息
Department of Bioengineering, University of Utah, Salt Lake City, UT 84112, USA.
出版信息
Acta Biomater. 2013 Jul;9(7):7200-8. doi: 10.1016/j.actbio.2013.02.047. Epub 2013 Mar 14.
Abstract
Surface-adsorbed fibrinogen (FBG) was recognized by adhering astrocytes, and was removed from the substrates in vitro by a two-phase removal process. The cells removed adsorbed FBG from binary proteins' surface patterns (FBG+laminin, or FBG+albumin) while leaving the other protein behind. Astrocytes preferentially expressed chondroitin sulfate proteoglycan (CSPG) at the loci of fibrinogen stimuli; however, no differences in overall CSPG production as a function of FBG surface coverage were identified. Removal of FBG by astrocytes was also found to be independent of transforming growth factor type β (TGF-β) receptor based signaling as cells maintained CSPG production in the presence of TGF-β receptor kinase inhibitor, SB 431542. The inhibitor decreased CSPG expression, but did not abolish it entirely. Because blood contact and subsequent FBG adsorption are unavoidable in neural implantations, the results indicate that implant-adsorbed FBG may contribute to reactive astrogliosis around the implant as astrocytes specifically recognize adsorbed FBG.
摘要
表面吸附的纤维蛋白原 (FBG) 被黏附的星形胶质细胞识别，并通过两相去除过程从体外的基质中去除。细胞从二元蛋白表面模式（FBG+层粘连蛋白或 FBG+白蛋白）中去除吸附的 FBG，而将另一种蛋白质留在后面。星形胶质细胞在纤维蛋白原刺激的部位优先表达硫酸软骨素蛋白聚糖 (CSPG)；然而，在纤维蛋白原表面覆盖率的功能方面，CSPG 的整体产生没有差异。星形胶质细胞对 FBG 的去除也被发现与转化生长因子-β (TGF-β) 受体信号无关，因为在 TGF-β 受体激酶抑制剂 SB 431542 的存在下，细胞维持 CSPG 的产生。抑制剂降低了 CSPG 的表达，但并没有完全消除它。由于在神经植入物中不可避免地会发生血液接触和随后的 FBG 吸附，因此这些结果表明，植入物吸附的 FBG 可能会导致植入物周围的反应性星形胶质细胞增生，因为星形胶质细胞特异性识别吸附的 FBG。
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本文引用的文献
1
Vascular damage in the central nervous system: a multifaceted role for vascular-derived TGF-β.中枢神经系统中的血管损伤：血管衍生的 TGF-β 的多方面作用。
Cell Tissue Res. 2012 Jan;347(1):187-201. doi: 10.1007/s00441-011-1228-0. Epub 2011 Aug 18.
2
Functional regeneration of respiratory pathways after spinal cord injury.脊髓损伤后呼吸道的功能再生。
Nature. 2011 Jul 13;475(7355):196-200. doi: 10.1038/nature10199.
3
Using microcontact printing of fibrinogen to control surface-induced platelet adhesion and activation.利用纤维蛋白原的微接触印刷术控制表面诱导的血小板黏附和激活。
Langmuir. 2011 Jul 5;27(13):8316-22. doi: 10.1021/la201064d. Epub 2011 Jun 9.
4
Fibrinogen triggers astrocyte scar formation by promoting the availability of active TGF-beta after vascular damage.纤维蛋白原通过促进血管损伤后活性 TGF-β的可用性触发星形胶质细胞瘢痕形成。
J Neurosci. 2010 Apr 28;30(17):5843-54. doi: 10.1523/JNEUROSCI.0137-10.2010.
5
Quantitative analysis of the tissue response to chronically implanted microwire electrodes in rat cortex.大鼠皮层内慢性植入微丝电极的组织反应的定量分析。
Biomaterials. 2010 Mar;31(7):1558-67. doi: 10.1016/j.biomaterials.2009.11.049. Epub 2009 Dec 5.
6
An in vitro model of the inhibition of axon growth in the lesion scar formed after central nervous system injury.中枢神经系统损伤后形成的病变瘢痕中抑制轴突生长的体外模型。
Mol Cell Neurosci. 2010 Feb;43(2):177-87. doi: 10.1016/j.mcn.2009.10.008. Epub 2009 Nov 6.
7
A versatile 3D culture model facilitates monitoring of astrocytes undergoing reactive gliosis.一种多功能的 3D 培养模型有助于监测发生反应性神经胶质增生的星形胶质细胞。
J Tissue Eng Regen Med. 2009 Dec;3(8):634-46. doi: 10.1002/term.209.
8
Molecular dissection of reactive astrogliosis and glial scar formation.解析反应性星形胶质细胞增生和神经胶质瘢痕形成。
Trends Neurosci. 2009 Dec;32(12):638-47. doi: 10.1016/j.tins.2009.08.002. Epub 2009 Sep 24.
9
Investigating the elusive mechanism of glycosaminoglycan biosynthesis.探究难以捉摸的糖胺聚糖生物合成机制。
J Biol Chem. 2009 Sep 18;284(38):25842-53. doi: 10.1074/jbc.M109.043208. Epub 2009 Jul 23.
10
Blood-brain barrier breakdown-inducing astrocytic transformation: novel targets for the prevention of epilepsy.血脑屏障破坏诱导的星形胶质细胞转化：预防癫痫的新靶点。
Epilepsy Res. 2009 Aug;85(2-3):142-9. doi: 10.1016/j.eplepsyres.2009.03.005. Epub 2009 Apr 11.
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