Laboratório de Microbiologia Ambiental, EMBRAPA Meio Ambiente, Rod SP 340-Km 127, 5, PO Box 69, 13820-000, Jaguariúna, Brazil.
Antonie Van Leeuwenhoek. 2013 Jun;103(6):1235-44. doi: 10.1007/s10482-013-9901-9. Epub 2013 Mar 16.
The taxonomic position of a streptomycete isolated from a potato tubercle was determined by using a polyphasic approach. The organism had chemotaxonomic and morphological properties consistent with its classification in the genus Streptomyces and formed a distinct phyletic line in the Streptomyces 16S rRNA gene tree. It was found to be closely related to Streptomyces celluloflavus NRRL B-2493(T) (99.4 % 16S rRNA gene similarity) and shared a 99.0 % 16S rRNA gene similarity value with Streptomyces albolongus NRRL B-3604(T) and Streptomyces cavourensis subsp. cavourensis NBRC 13026(T); low levels of DNA-DNA relatedness with these organisms showed that the isolate belonged to a distinct genomic species. The isolate was distinguished readily from the type strains of these species using a combination of morphological and other phenotypic properties. On the basis of these results, it is proposed that isolate ASBV-1(T) (= CBMAI 1465(T) = CCMA 894(T) = NRRL B-24922(T)) be classified as the type strain of Streptomyces araujoniae sp. nov.
采用多相分类法确定了从土豆块茎中分离出的链霉菌的分类地位。该生物具有与链霉菌属分类一致的化学生态分类学和形态学特性，并在链霉菌 16S rRNA 基因树中形成了一个独特的系统发育谱系。它与链霉菌纤维素黄质 NRRL B-2493(T)（99.4% 16S rRNA 基因相似度）密切相关，与链霉菌白长孢变种 NRRL B-3604(T)和链霉菌卡沃氏亚种 NRRL B-3604(T)的 16S rRNA 基因相似度值为 99.0%；与这些生物的 DNA-DNA 同源性较低表明该分离株属于一个独特的基因组种。该分离株在形态和其他表型特性上与这些种的模式菌株有明显的区别。基于这些结果，建议将分离株 ASBV-1(T)（=CBMAI 1465(T)=CCMA 894(T)=NRRL B-24922(T)）归类为新种链霉菌 araujoniae 的模式菌株。
