Department of Microbiology (ZIEL), Technische Universität München, Weihenstephaner Berg 3, D-85350 Freising, Germany.
Int J Syst Evol Microbiol. 2013 Sep;63(Pt 9):3358-3363. doi: 10.1099/ijs.0.051565-0. Epub 2013 Mar 15.
A Gram-stain-positive, strictly aerobic, rod-shaped and weakly motile bacterium, designated WS 4627(T), was isolated from a dairy product sample collected in southern Germany. Spherical to slightly ellipsoidal endospores were formed centrally or subterminally in sometimes slightly swollen sporangia. The isolate was able to grow at 8-35 °C, at pH 6.5-8.5 and with 0.5-16.5% (w/v) NaCl. The diamino acid of the cell wall was meso-diaminopimelic acid (peptidoglycan type A1γ) and the genomic DNA G+C content was 39.1 mol%. The major menaquinone was MK-7, the cellular fatty acid profile contained major amounts of anteiso-C(15:0) and anteiso-C(17:0) and the major polar lipids were diphosphatidylglycerol and phosphatidylglycerol. Strain WS 4627(T) was most closely related to 'Virgibacillus natachei' FarD (96.5% 16S rRNA gene sequence similarity) and 'Virgibacillus zhanjiangensis' JSM 079157 (96.0%). Based on the data presented, strain WS 4627(T) represents a novel species of the genus Virgibacillus, for which the name Virgibacillus halotolerans sp. nov. is proposed. The type strain is WS 4627(T) ( =DSM 25060(T) =LMG 26644(T)).
一株革兰氏阳性、严格需氧、杆状且弱运动的细菌，被命名为 WS 4627(T)，从德国南部采集的乳制品样本中分离得到。内生孢子呈球形至稍椭圆形，中央或亚末端形成，有时在稍膨胀的孢子囊中。该分离株能够在 8-35°C、pH 值 6.5-8.5 和 0.5-16.5%（w/v）NaCl 的条件下生长。细胞壁的二氨基庚二酸是meso-二氨基庚二酸（肽聚糖类型 A1γ），基因组 DNA G+C 含量为 39.1 mol%。主要的menaquinone 是 MK-7，细胞脂肪酸图谱含有大量的 anteiso-C(15:0)和 anteiso-C(17:0)，主要的极性脂是双磷脂酰甘油和磷脂酰甘油。WS 4627(T)菌株与 'Virgibacillus natachei' FarD（16S rRNA 基因序列相似度为 96.5%）和 'Virgibacillus zhanjiangensis' JSM 079157（96.0%）最为密切相关。基于所提供的数据，WS 4627(T)菌株代表了 Virgibacillus 属的一个新种，因此提议将其命名为耐盐性 Virgibacillus halotolerans sp. nov.。该模式菌株为 WS 4627(T)（=DSM 25060(T) = LMG 26644(T)）。
