3B's Research Group-Biomaterials, Biodegradables and Biomimetics, University of Minho, Guimarães, Portugal; ICVS/3B's-PT Government Associate Laboratory, Braga/Guimarães, Portugal.
J Biomed Mater Res A. 2013 Nov;101(11):3059-65. doi: 10.1002/jbm.a.34614. Epub 2013 Mar 18.
Osteoblast-like cells together with a suitable scaffold can aid to the regeneration of bone defects. A suitable scaffold could be starch poly(ε-caprolactone) (SPCL) fiber meshes, which have shown a high potential to support bone formation in previous in vitro and in noncritical sized in vivo studies. The aim of this study was to assess the effect of these scaffolds alone or combined with osteoblast-like cells in the regeneration of a critical-sized cranial defect in male Fisher rats. Empty defects and defects filled with cell-free scaffolds were used as controls groups. Samples were analyzed by microcomputed tomography (micro-CT) and histological analyses. Histological analyses revealed that all study groups showed new bone formation from the defect edges toward the interior of the defects. In addition, bone was formed in the center of the scaffolds, especially in the groups containing preloaded osteoblast-like cells. Micro-CT reconstructions showed that bone formation increased over time and was enhanced with the inclusion of preloaded osteoblast-like cells compared with SPCL scaffolds alone. According to these results, the preloaded osteoblast-like cells contributed to the bone regeneration process in a critical-sized bone defect. Furthermore, SPCL fiber meshes proved to be an osteoconductive material to use for bone regeneration purposes.
成骨样细胞与合适的支架一起可以帮助骨缺损的再生。合适的支架可以是淀粉聚己内酯（SPCL）纤维网，在之前的体外和非临界大小的体内研究中，它显示出了很高的支持骨形成的潜力。本研究的目的是评估这些支架单独或与成骨样细胞一起在雄性 Fisher 大鼠临界大小的颅骨缺损中的再生效果。空缺陷和不含细胞的支架填充缺陷被用作对照组。通过微计算机断层扫描（micro-CT）和组织学分析对样本进行分析。组织学分析表明，所有研究组均显示出从缺陷边缘向缺陷内部形成新骨。此外，在支架的中心形成了骨，特别是在含有预加载的成骨样细胞的组中。微 CT 重建显示，随着时间的推移，骨形成增加，与单独使用 SPCL 支架相比，包含预加载的成骨样细胞的组中的骨形成得到了增强。根据这些结果，预加载的成骨样细胞有助于临界大小骨缺损中的骨再生过程。此外，SPCL 纤维网被证明是一种用于骨再生目的的骨传导材料。
