Brinkman E, Poot A, van der Does L, Bantjes A
Department of Chemical Technology, Twente University of Technology, Enschede, The Netherlands.
Biomaterials. 1990 Apr;11(3):200-5. doi: 10.1016/0142-9612(90)90156-k.
Pellethane 2363 80A films and tubings were chemically modified and the effect of these modifications on platelet deposition was studied. Grafting of high molecular weight poly(ethylene oxide) and graft polymerization of methoxy poly(ethylene glycol) 400 methacrylate resulted in surfaces with a good water wettability. The increased hydrophilicity of these modified surfaces could be demonstrated by contact angle measurements. The platelet deposition was investigated with tubings in a capillary flow system, using different types of perfusates. Platelet deposition from a buffer-containing perfusate on surfaces modified with either high molecular weight poly(ethylene oxide) or methoxy poly(ethylene glycol) 400 methacrylate was almost absent and less than on Pellethane 2363 80A. Using a citrated plasma-containing perfusate the amount of deposited platelets on Pellethane 2363 80A modified with high molecular weight poly(ethylene oxide) was low and about the same as on unmodified surfaces. However, a marked reduced platelet deposition compared to unmodified Pellethane 2363 80A was found when the platelets were activated by Ca2+ ionophore. The improved blood compatibility of the modified Pellethane 2363 80A tubings obviously indicates the favourable effect of the presence of grafted PEO on the surface.
对聚醚氨酯2363 80A薄膜和管材进行了化学改性，并研究了这些改性对血小板沉积的影响。接枝高分子量聚环氧乙烷以及甲基丙烯酸甲氧基聚乙二醇400的接枝聚合反应产生了具有良好水润湿性的表面。这些改性表面亲水性的增强可通过接触角测量得到证明。在毛细管流动系统中使用不同类型的灌注液，通过管材研究血小板沉积情况。在由高分子量聚环氧乙烷或甲基丙烯酸甲氧基聚乙二醇400改性的表面上，含缓冲液的灌注液中的血小板沉积几乎不存在，且比在聚醚氨酯2363 80A上更少。使用含枸橼酸盐血浆的灌注液时，用高分子量聚环氧乙烷改性的聚醚氨酯2363 80A上沉积的血小板数量较少，与未改性表面上的情况大致相同。然而，当血小板被钙离子载体激活时，与未改性的聚醚氨酯2363 80A相比，发现血小板沉积明显减少。改性的聚醚氨酯2363 80A管材血液相容性的改善明显表明表面接枝聚环氧乙烷的存在具有有利作用。
