Egert G, Kanz L, Löhr G W, Fauser A A
Department of Hematology, Medical School, Albert-Ludwigs-University, Freiburg, Federal Republic of Germany.
Blut. 1990 May;60(5):282-6. doi: 10.1007/BF01736229.
Studies have shown that recombinant human alpha interferon (rIFN alpha) inhibits the growth of colonies of multipotential stem cells from human bone marrow. This report demonstrates that rIFN alpha inhibits the growth of such colonies from the bone marrow of patients with chronic myelogenous leukemia (CML) to a greater extent than from bone marrow of healthy individuals. It also shows that T lymphocyte colonies subcloned with interleukin 2 (IL-2) from CML mixed colonies were inhibited more by rIFN alpha than were similar colonies subcultured from normal mixed colonies. The report demonstrates that the Ph' chromosome is present in such T cell colonies subcultured from CML mixed colonies. When mixed colonies were grown from CML bone marrow in the presence of rIFN alpha, Ph' negative colonies were observed, whereas no such Ph' negative mixed colonies grew from a similar number of bone marrow cells incubated without rIFN alpha. These observations confirm that T lymphocytes derived from bone marrow stem cells are from the CML clone, and that the inhibition of growth of Ph' positive colonies, by rIFN alpha permits the growth of residual normal stem cells. The disappearance of the Ph-chromosome in subclones of T lymphocytes supports the notion of nonclonal hematopoiesis in patients with CML.
研究表明，重组人α干扰素（rIFNα）可抑制人骨髓多能干细胞集落的生长。本报告表明，rIFNα对慢性粒细胞白血病（CML）患者骨髓中此类集落生长的抑制作用比对健康个体骨髓的抑制作用更强。报告还显示，从CML混合集落中用白细胞介素2（IL-2）亚克隆的T淋巴细胞集落比从正常混合集落传代培养的类似集落更易被rIFNα抑制。该报告表明，从CML混合集落传代培养的此类T细胞集落中存在Ph'染色体。当在rIFNα存在的情况下从CML骨髓中培养混合集落时，观察到了Ph'阴性集落，而在没有rIFNα的情况下培养相同数量的骨髓细胞则未长出此类Ph'阴性混合集落。这些观察结果证实，源自骨髓干细胞的T淋巴细胞来自CML克隆，并且rIFNα对Ph'阳性集落生长的抑制作用使得残留的正常干细胞得以生长。T淋巴细胞亚克隆中Ph染色体的消失支持了CML患者非克隆性造血的观点。
