脑心通对动脉粥样硬化兔动脉粥样硬化及诱导型一氧化氮合酶表达的影响。

Effects of Naoxintong on atherosclerosis and inducible nitric oxide synthase expression in atherosclerotic rabbit.

机构信息

Department of Neurology, Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong 510630, China.

出版信息

Chin Med J (Engl). 2013 Mar;126(6):1166-70.

Abstract

BACKGROUND

High levels of nitric oxide (NO) produced by inducible NO synthase (iNOS) have been associated with atherosclerosis processes. Naoxintong is a traditional Chinese medicine for treatment of cerebrovascular and cardiovascular disease. The aim of the present study was to detect and quantify changes of iNOS mRNA and NO levels in the vessel wall after the administration of Naoxintong in an atherosclerotic rabbit model.

METHODS

Forty New Zealand white rabbits were randomly divided into five groups (n = 8). Rabbits were fed a standard diet (group A), an atherogenic diet consisting of 79% standard feed + 1% cholesterol + 5% lard + 15% egg yolk powder (group B), an atherogenic diet with Naoxintong 0.25 mg×kg(-1)×d(-1) (group C), an atherogenic diet with Naoxintong 0.5 mg×kg(-1)×d(-1) (group D), or atherogenic diet with Naoxintong 1.0 mg×kg(-1)×d(-1) (group E) for 12 weeks.

RESULTS

Supplemented administration of Naoxintong led to a down-regulation of cholesterol (CHOL) (P < 0.001) and low-density lipoprotein (LDL) (P < 0.001). The trend became more notable as the dose of Naoxintong increased; group C vs. group B (CHOL, P = 0.568; LDL-cholesterol (LDL-C), P = 0.119), group D vs. group B (CHOL, P = 0.264; LDL-C, P = 0.027), group E vs. group B (CHOL, P = 0.028; LDL-C, P = 0.002). Atherosclerotic lesions in aorta were reduced in Naoxintong groups (groups C, D, E) compared to group B. Group B had higher iNOS mRNA (P = 0.001) and NO level (P < 0.001) than group A. Compared with the atherogenic diet fed-rabbits, Naoxintong supplements decreased the expression of iNOS mRNA (P < 0.001) and the NO level (P < 0.001) in the vessel wall. Groups given a higher Naoxintong dose exhibited greater benefits. iNOS mRNA and NO levels seemed to be reduced in group C, although the difference did not quite reach statistical significance (iNOS mRNA, P = 0.130; NO, P = 0.038). iNOS mRNA and NO levels significantly decreased in group D (iNOS mRNA, P = 0.019; NO, P = 0.018) and group E (iNOS mRNA, P = 0.004; NO, P < 0.001).

CONCLUSION

Naoxintong has beneficial effects on atherosclerosis treatment by reducing expression of iNOS mRNA and the NO level in the vessel wall.

摘要

背景

诱导型一氧化氮合酶（iNOS）产生的高水平一氧化氮（NO）与动脉粥样硬化过程有关。脑心通是一种治疗心脑血管疾病的中药。本研究旨在检测并量化在动脉粥样硬化兔模型中给予脑心通后血管壁中 iNOS mRNA 和 NO 水平的变化。

方法

40 只新西兰白兔随机分为五组（n = 8）。兔子分别喂食标准饮食（A 组）、含 79%标准饲料+1%胆固醇+5%猪油+15%蛋黄粉的致动脉粥样硬化饮食（B 组）、含 0.25 mg·kg-1·d-1 脑心通的致动脉粥样硬化饮食（C 组）、含 0.5 mg·kg-1·d-1 脑心通的致动脉粥样硬化饮食（D 组）或含 1.0 mg·kg-1·d-1 脑心通的致动脉粥样硬化饮食（E 组），持续 12 周。

结果

脑心通的补充给药导致胆固醇（CHOL）（P < 0.001）和低密度脂蛋白（LDL）（P < 0.001）的下调。随着脑心通剂量的增加，这种趋势变得更加明显；C 组与 B 组相比（CHOL，P = 0.568；LDL-胆固醇（LDL-C），P = 0.119），D 组与 B 组相比（CHOL，P = 0.264；LDL-C，P = 0.027），E 组与 B 组相比（CHOL，P = 0.028；LDL-C，P = 0.002）。与 B 组相比，脑心通组（C、D、E 组）主动脉粥样硬化病变减少。B 组的 iNOS mRNA（P = 0.001）和 NO 水平（P < 0.001）均高于 A 组。与喂食致动脉粥样硬化饮食的兔子相比，脑心通补充剂降低了血管壁中 iNOS mRNA（P < 0.001）和 NO 水平（P < 0.001）的表达。给予更高剂量脑心通的组表现出更大的益处。尽管差异尚未达到统计学意义（iNOS mRNA，P = 0.130；NO，P = 0.038），但 C 组的 iNOS mRNA 和 NO 水平似乎有所降低。D 组（iNOS mRNA，P = 0.019；NO，P = 0.018）和 E 组（iNOS mRNA，P = 0.004；NO，P < 0.001）的 iNOS mRNA 和 NO 水平显著降低。