Yuan Shuping, Jin Jianhua, Chen Lu, Hou Yongzhong, Wang Hong
Institute of Life Sciences, Jiangsu University, Zhenjiang, Jiangsu 212013, China.
Department of Oncology, Affiliated Wujin People's Hospital, Jiangsu University, Changzhou 212017, China.
Evid Based Complement Alternat Med. 2017;2017:3801976. doi: 10.1155/2017/3801976. Epub 2017 Feb 15.
As a traditional Chinese medicine, Naoxintong capsule (NXT) has been approved by China Food and Drug Administration (CFDA), which is used for cardiocerebrovascular disease treatment. Here we found that NXT extract significantly promoted H9c2 cardiomyocyte cell autophagy involved in increased autophagy-associated gene expression leading to inhibition of mTOR signaling. Moreover, NXT extract increased PPAR protein expression and transcription activity of H9c2 cell. Consistent with this, in PPAR gene silenced H9c2 cells, NXT had no effect on autophagy and mTOR signaling. Furthermore, NXT/PPAR-mediated H9c2 autophagy led to inhibition of cardiomyocyte cell hypertrophy. These findings suggest that the extract of NXT inhibited H9c2 cardiomyocyte cell hypertrophy via PPAR-mediated cell autophagy.
作为一种传统中药，脑心通胶囊（NXT）已获中国食品药品监督管理总局（CFDA）批准，用于治疗心脑血管疾病。在此我们发现，NXT提取物显著促进H9c2心肌细胞自噬，这涉及自噬相关基因表达增加，进而导致mTOR信号传导受到抑制。此外，NXT提取物增加了H9c2细胞的PPAR蛋白表达和转录活性。与此一致的是，在PPAR基因沉默的H9c2细胞中，NXT对自噬和mTOR信号传导没有影响。此外，NXT/PPAR介导的H9c2自噬导致心肌细胞肥大受到抑制。这些发现表明，NXT提取物通过PPAR介导的细胞自噬抑制H9c2心肌细胞肥大。
