西洋参对环磷酰胺处理大鼠附睾精子分析的有益作用。
Beneficial effects of american ginseng on epididymal sperm analyses in cyclophosphamide treated rats.
机构信息
1. Reaserch and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran ; 2. Department of Biology, Faculty of Science, University of Urmia, Urmia, Iran.
出版信息
Cell J. 2012 Summer;14(2):116-21. Epub 2012 Aug 31.
OBJECTIVE
This study aims to evaluate the protective effects of American ginseng administered by gastric intubation on sperm vital quality in adult male rats treated with cyclophosphamide (CP).
MATERIALS AND METHODS
In this experimental study, 28 Adult male Wistar rats were assigned to four groups, seven rats in each. The animals allocated to control, CP treated, Ginseng treated and CP-Ginseng treated groups. Rats were treated with CP (6.1 mg/kg/day, i.p) for 6 weeks. American ginseng was used at a dose of 500 mg/kg/day during treatment. Sperm analysis (motion, count, morphology and viability) were evaluated at the end of the experiments. Sperm motion was assessed by Computer-Assisted Sperm Analysis (CASA). The data were analyzed using GB stat software. Probability values of p<0.05 and p<0.01 were considered significant.
RESULTS
The epididymal sperm counts in the groups that received CP showed significant decreases compared to the control group. Also dead and abnormal sperms significantly increased following CP treatment compared with control. The motility of caudal sperm was reduced significantly with CP treatment. Therefore, according to the results of this study, co-administration of CP and American ginseng can improve these parameters.
CONCLUSION
American ginseng can prevent the cytotoxic effects of CP on sperm quality factors.
目的
本研究旨在评估通过胃管给予西洋参对环磷酰胺（CP）处理的成年雄性大鼠精子活力的保护作用。
材料与方法
在这项实验研究中，将 28 只成年雄性 Wistar 大鼠分为四组，每组 7 只。将动物分为对照组、CP 处理组、人参处理组和 CP-人参处理组。CP（6.1mg/kg/天，腹腔内注射）治疗 6 周。在治疗期间，西洋参的用量为 500mg/kg/天。实验结束时，评估精子分析（运动、计数、形态和活力）。通过计算机辅助精子分析（CASA）评估精子运动。使用 GB stat 软件分析数据。p<0.05 和 p<0.01 的概率值被认为具有统计学意义。
结果
与对照组相比，接受 CP 治疗的附睾精子计数明显减少。与对照组相比，CP 处理后死精和畸形精子显著增加。尾部精子的活力也明显降低。因此，根据这项研究的结果，CP 和西洋参的联合给药可以改善这些参数。
结论
西洋参可以预防 CP 对精子质量因素的细胞毒性作用。
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