Department of Neurosurgery, Ipekyolu State Hospital, 65300, Van, Turkey.
Acta Neurochir (Wien). 2013 May;155(5):913-22. doi: 10.1007/s00701-013-1672-6. Epub 2013 Mar 20.
The purpose of our study was to investigate the effect of ginseng on antioxidant enzyme levels in brain damage following experimental diffuse head trauma in rats. The neuroprotective effect of ginseng was also studied.
In this study, rats were divided into four groups, and the rats in group 1 received no intervention. In group 2, the rats were administered 50 mg/kg ginseng, injected intraperitoneally at 1, 24 and 48 h, and the effect of ginseng on normal tissues was studied. No drugs were administered to the rats in group 3 who had previously experienced diffuse head trauma using Feeney's falling weight method. In group 4, rats underwent Feeney's falling weight method, leading to diffuse head trauma, and they were given 50 mg/kg ginseng intraperitoneally 1, 24 and 48 h after head trauma. Rats were killed 72 h after head trauma and their brain tissues extracted for histopathological and biochemical studies.
Histopathological study of brain cross sections in the trauma group demonstrated neurons in the trauma region and surrounding area, which generally had a dark-colored eosinophilic cytoplasm and a pyknotic nucleus, while the nuclei of neurons were located peripherally. However, brain cross sections in group 4 from rats given ginseng after head trauma showed fewer neurons with eosinophilic cytoplasm, pyknotic and peripheral nuclei in the trauma region and surrounding area. No statistically significant difference in the tissue SOD level was observed; however, the GSH Px level in group 4 was significantly reduced compared to that in group 3.
After affecting the GSH Px level and reducing histopathological scores, ginseng was found to display antioxidant and neuroprotective activity.
本研究旨在探讨人参对实验性弥漫性颅脑损伤后大鼠脑损伤中抗氧化酶水平的影响，并研究人参的神经保护作用。
本研究将大鼠分为 4 组，第 1 组大鼠未进行任何干预，第 2 组大鼠于伤后 1、24 和 48 小时分别给予 50mg/kg 人参，腹腔注射，研究人参对正常组织的影响，第 3 组大鼠采用 Feeney 落体法造成弥漫性颅脑损伤，未给予任何药物，第 4 组大鼠采用 Feeney 落体法造成弥漫性颅脑损伤，伤后 1、24 和 48 小时分别给予 50mg/kg 人参腹腔注射。伤后 72 小时处死大鼠，提取脑组织进行组织病理学和生物化学研究。
创伤组脑切片的组织病理学研究显示，创伤区域和周围区域的神经元通常具有深染的嗜酸性细胞质和固缩核，而神经元的核位于周围。然而，给予人参后创伤组 4 的大鼠脑切片显示，创伤区域和周围区域的神经元具有较少的嗜酸性细胞质、固缩核和周围核。组织 SOD 水平无统计学差异；然而，与第 3 组相比，第 4 组 GSH Px 水平显著降低。
人参通过影响 GSH Px 水平和降低组织病理学评分，显示出抗氧化和神经保护作用。
