Song Meiyun, Chen Yun, Du Hongxu, Zhang Shuaibing, Wang Yixuan, Zeng Ling, Yang Jingjing, Shi Jintong, Wu Yi, Wang Deyun, Hu Yuanliang, Liu Jiaguo
Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, P R China.
Afr J Tradit Complement Altern Med. 2017 Jun 5;14(4):8-21. doi: 10.21010/ajtcam.v14i4.2. eCollection 2017.
Duck viral hepatitis (DVH), caused by duck hepatitis A virus (DHAV), is a fatal contagious infectious disease which spreads rapidly with high morbidity and high mortality, and there is no effective clinical drug against DVH.
Raw Rehmannia Radix Polysaccharide (RRRP), Lycii Fructus polysaccharides and Astragalus Radix polysaccharides were experimented in vitro and in vivo. Mortality rate, livers change, liver lesion scoring, peroxidative injury evaluation indexes in vitro and in vivo, and hepatic injury evaluation indexes of optimal one were detected and observed in this experiment.
RRRP could reduce mortality with the protection rate about 20.0% compared with that of the viral control (VC) group, finding that RRRP was the most effective against DHAV. The average liver scoring of the VC, blank control (BC), RRRP groups were 3.5, 0, 2.1. Significant difference (<0.05) appeared between any two groups, demonstrating that it can alleviate liver pathological change. RRRP could make the hepatic injury evaluation indexes similar to BC group while the levels of the VC group were higher than other two groups in general. The levels of SOD, GSH-Px, CAT of RRRP group showed significant higher than that of VC group while the levels of NOS and MDA showed the opposite tendency, thus, RRRP could release peroxidative injury.
RRRP was the most effective against duck hepatitis A virus (DHAV). RRRP could reduce mortality, alleviate liver pathological change, down-regulate liver lesion score, release peroxidative injury and hepatic injury. The antiviral and peroxidative injury releasing activity of RRRP for DHAV provided a platform to test novel drug strategies for hepatitis A virus in human beings.
鸭病毒性肝炎（DVH）由鸭甲型肝炎病毒（DHAV）引起，是一种致命的传染性疾病，传播迅速，发病率和死亡率高，且尚无有效的临床药物治疗DVH。
对生地黄多糖（RRRP）、枸杞多糖和黄芪多糖进行体内外实验。本实验检测并观察了死亡率、肝脏变化、肝脏病变评分、体内外过氧化损伤评价指标以及最佳药物的肝损伤评价指标。
RRRP可降低死亡率，与病毒对照组（VC）相比保护率约为20.0%，发现RRRP对DHAV最有效。VC组、空白对照组（BC）、RRRP组的平均肝脏评分分别为3.5、0、2.1。任意两组之间均出现显著差异（<0.05），表明其可减轻肝脏病理变化。RRRP可使肝损伤评价指标与BC组相似，而VC组的指标总体高于其他两组。RRRP组的超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）、过氧化氢酶（CAT）水平显著高于VC组，而一氧化氮合酶（NOS）和丙二醛（MDA）水平则呈相反趋势，因此，RRRP可减轻过氧化损伤。
RRRP对鸭甲型肝炎病毒（DHAV）最有效。RRRP可降低死亡率，减轻肝脏病理变化，下调肝脏病变评分，减轻过氧化损伤和肝损伤。RRRP对DHAV的抗病毒和减轻过氧化损伤活性为测试人类甲型肝炎病毒的新型药物策略提供了平台。
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