Swida U, Lucka L, Kress H
Institut für Allgemeine Genetik, FU Berlin, FRG.
Development. 1990 Feb;108(2):269-80. doi: 10.1242/dev.108.2.269.
The gene Lgp-1, which is localized in the intermoult puff 16A of D. virilis polytene chromosomes, encodes the major larval glue protein Igp-1. The gene consists of two exons interrupted by a short intron. In the 5' flanking region of Lgp-1, we find putative ecdysone receptor binding sites and two proximal conserved sequence motifs which are possibly involved in gene regulation. The amino acid sequence deduced from the DNA sequence reveals a relationship to the 68C glue protein family of D. melanogaster. The size of the Lgp-1 transcripts decreases in late third instar larvae concomitantly with their disappearance. This is caused by deadenylation followed by distinct nucleolytic attacks in the 3' untranslated region. Preliminary data suggest the presence of another glue protein gene in the 16A puff region which is related to the Lgp-1 gene.
基因Lgp - 1定位于果蝇（D. virilis）多线染色体的蜕皮间期胀泡16A中，编码主要的幼虫黏蛋白Igp - 1。该基因由两个外显子组成，中间被一个短内含子隔开。在Lgp - 1的5'侧翼区域，我们发现了假定的蜕皮激素受体结合位点和两个可能参与基因调控的近端保守序列基序。从DNA序列推导的氨基酸序列显示与黑腹果蝇（D. melanogaster）的68C黏蛋白家族存在关联。Lgp - 1转录本的大小在三龄后期幼虫中减小，同时它们消失。这是由多聚腺苷酸化缩短随后在3'非翻译区发生明显的核酸酶攻击所导致的。初步数据表明在16A胀泡区域存在另一个与Lgp - 1基因相关的黏蛋白基因。
