Tissue Engineering Group-TEG, National Orthopaedic Centre for Research and Learning-NOCERAL, Department of Orthopaedic Surgery, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
J Mater Sci Mater Med. 2013 Jun;24(6):1561-70. doi: 10.1007/s10856-013-4907-4. Epub 2013 Mar 20.
This study aims to pre-assess the in vitro and in vivo biocompatibility of poly(vinyl alcohol)-carboxylmethyl-chitosan-poly(ethylene glycol) (PCP) scaffold. PCP was lyophilised to create supermacroporous structures. 3-(4, 5-dimethyl-thiazol-2yl)-2, 5-diphenyltetrazolium bromide (MTT) assay and immunohistochemistry (IHC) were used to evaluate the effectiveness of PCP scaffolds for chondrocytes attachment and proliferation. The ultrastructural was assessed using scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Extracellular matrix (ECM) formation was evaluated using collagen type-II staining, glycosaminoglycan (GAG) and collagen assays. Histological analysis was conducted on 3-week implanted Sprague-Dawley rats. The MTT, IHC, SEM and TEM analyses confirm that PCP scaffolds promoted cell attachment and proliferation in vitro. The chondrocyte-PCP constructs secreted GAG and collagen type-II, both increased significantly from day-14 to day-28 (P < 0.05). PCP scaffolds did not elicit any adverse effects on the host tissue, but were partially degraded. These results suggest that supermacroporous PCP is a biocompatible scaffold for clinical applications.
本研究旨在预先评估聚乙烯醇-羧甲基壳聚糖-聚乙二醇（PCP）支架的体外和体内生物相容性。PCP 经冷冻干燥制成超大孔结构。3-(4,5-二甲基噻唑-2 基)-2,5-二苯基四氮唑溴盐（MTT）测定和免疫组织化学（IHC）用于评估 PCP 支架对软骨细胞附着和增殖的效果。使用扫描电子显微镜（SEM）和透射电子显微镜（TEM）评估超微结构。使用 II 型胶原染色、糖胺聚糖（GAG）和胶原测定评估细胞外基质（ECM）的形成。对植入 Sprague-Dawley 大鼠 3 周后的组织进行了组织学分析。MTT、IHC、SEM 和 TEM 分析证实 PCP 支架在体外促进了细胞附着和增殖。软骨细胞-PCP 构建体分泌 GAG 和 II 型胶原，从第 14 天到第 28 天显著增加（P<0.05）。PCP 支架对宿主组织没有任何不良影响，但部分降解。这些结果表明，超大孔 PCP 是一种用于临床应用的生物相容性支架。
