Department of Molecular Science and Technology, Ajou University, Suwon 443-749, Korea.
J Biomed Mater Res A. 2012 Jul;100(7):1751-60. doi: 10.1002/jbm.a.34135. Epub 2012 Mar 29.
In this work, the in vivo biodegradation of, biocompatibility of, and host response to various topographic scaffolds were investigated. Randomly oriented fibrous poly(L-lactide) (PLLA) nanofibers were fabricated using the electrospinning technique. A PLLA scaffold was obtained by salt leaching. Both the electrospun PLLA nanofibers and the salt-leaching PLLA scaffolds formed three-dimensional pore structures. Cytotoxicity studies, in which rat muscle-derived stem cells (rMDSCs) were grown on electrospun PLLA nanofibers or the salt-leaching PLLA scaffolds, revealed that the rMDSCs cell count on the PLLA nanofibers was slightly higher than that on the salt-leaching PLLA scaffolds. An in vivo study was carried out by implanting the scaffolds subcutaneously into rats to test the biodegradation, biocompatibility, and host response at regular intervals over 0-4 weeks. The degradation of the PLLA nanofibers 1, 2, and 4 weeks after initial implantation was more extensive than that observed for the salt-leaching PLLA scaffolds. PLLA nanofibers seeded the growth of larger fibrous tissue masses due to in vivo cellular infiltration into the randomly oriented fibrillar structures of the PLLA nanofibers. In addition, the inflammatory cell accumulation in PLLA nanofibers was lower than that in the salt-leaching PLLA scaffolds. These results indicate that the electrospun PLLA nanofibers may serve as a good scaffold to elicit fibrous cellular infiltration, to minimize host response, and to enhance tissue-scaffold integration.
在这项工作中，研究了不同形貌支架的体内生物降解性、生物相容性和宿主反应。采用静电纺丝技术制备了各向同性的聚 L-乳酸（PLLA）纳米纤维。通过盐溶法得到 PLLA 支架。电纺 PLLA 纳米纤维和盐溶 PLLA 支架均形成了三维多孔结构。细胞毒性研究表明，在电纺 PLLA 纳米纤维或盐溶 PLLA 支架上培养大鼠肌肉源性干细胞（rMDSCs）时，rMDSCs 在 PLLA 纳米纤维上的细胞计数略高于盐溶 PLLA 支架上的细胞计数。通过将支架皮下植入大鼠体内进行体内研究，以在 0-4 周的时间内定期测试降解、生物相容性和宿主反应。与盐溶 PLLA 支架相比，初始植入后 1、2 和 4 周时 PLLA 纳米纤维的降解更为广泛。由于体内细胞渗透到 PLLA 纳米纤维的各向同性纤维结构中，PLLA 纳米纤维促进了更大纤维组织块的生长。此外，PLLA 纳米纤维中的炎症细胞积聚低于盐溶 PLLA 支架。这些结果表明，电纺 PLLA 纳米纤维可用作一种良好的支架，以引发纤维细胞浸润，最小化宿主反应，并增强组织-支架整合。
