Ragni G, Caccamo A M, Dalla Serra A, Guercilena S
University of Milan, Italy.
Fertil Steril. 1990 Jun;53(6):1072-5. doi: 10.1016/s0015-0282(16)53588-2.
Two methods of freezing semen taken from patients with testicular tumors or Hodgkin's disease before treatment were compared. Many patients already have semen abnormalities, so an optimal method is extremely important. Ejaculates from 8 patients with testicular tumors and 20 with Hodgkin's disease were frozen by fast-freezing or by slow-staged freezing. Effects of motility, viability, and swelling after thawing were significantly impaired with both methods. However, cryosurvival was better after slow- than fast-freezing: motility 24% +/- 12.4% versus 15% +/- 11.2%; viability 24.1% +/- 11.4% versus 17.3% +/- 10.4%, swelling 33.3% +/- 11% versus 27.6% +/- 12.8%. The effects were equal for normal and abnormal sperm. Sperm from tumor patients should be frozen by slow-staged freezing method in spite of the higher cost and longer time.
比较了两种在治疗前冷冻取自睾丸肿瘤患者或霍奇金病患者精液的方法。许多患者已经存在精液异常，因此一种最佳方法极为重要。来自8例睾丸肿瘤患者和20例霍奇金病患者的射精精液通过快速冷冻或缓慢分步冷冻进行冷冻。两种方法解冻后的活力、生存能力和肿胀效果均受到显著损害。然而，缓慢冷冻后的冷冻存活率优于快速冷冻：活力分别为24%±12.4%和15%±11.2%；生存能力分别为24.1%±11.4%和17.3%±10.4%，肿胀分别为33.3%±11%和27.6%±12.8%。正常和异常精子的效果相同。尽管成本较高且时间较长，但肿瘤患者的精子应采用缓慢分步冷冻法进行冷冻。
