Batista Diego Felipe Alves, de Freitas Neto Oliveiro Caetano, Lopes Priscila Diniz, de Almeida Adriana Maria, Barrow Paul Andrew, Berchieri Angelo
Departamento de Patologia Veterinária, Univ Estadual Paulista, São Paulo, Brazil.
J Vet Diagn Invest. 2013 Mar;25(2):259-62. doi: 10.1177/1040638713479361.
Salmonella Pullorum and Salmonella Gallinarum are classified as biovars of Salmonella enterica subsp. enterica serovar Gallinarum. These salmonellae are the causative agents of Pullorum disease and fowl typhoid, respectively, and are widely distributed throughout the world. Although many developed countries have eradicated these diseases from commercial poultry, they are still the cause of significant economic loss in developing countries. When serovar Gallinarum is isolated, it is difficult to immediately differentiate between biovars because they are antigenically identical by serotyping. However, they cause distinct diseases with different epidemiology, and therefore it is important to differentiate them. This may be done biochemically but takes 2 to 3 days. In the present study, S. Pullorum and S. Gallinarum whole genomes were compared, and 1 genomic region of difference, which is part of the ratA gene, was chosen as a molecular marker for a polymerase chain reaction assay to differentiate rapidly between these organisms. In all, 26 strains of S. Gallinarum and 17 S. Pullorum strains were tested and successfully differentiated by the assay.
鸡白痢沙门氏菌和鸡伤寒沙门氏菌被归类为肠炎沙门氏菌亚种肠炎血清型鸡伤寒沙门氏菌的生物变种。这些沙门氏菌分别是鸡白痢和禽伤寒的病原体，在世界各地广泛分布。尽管许多发达国家已在家禽商业养殖中根除了这些疾病，但它们仍是发展中国家重大经济损失的原因。当分离到鸡伤寒血清型时，很难立即区分生物变种，因为通过血清分型它们在抗原性上是相同的。然而，它们会引发具有不同流行病学特征的不同疾病，因此区分它们很重要。这可以通过生化方法完成，但需要2到3天。在本研究中，对鸡白痢沙门氏菌和鸡伤寒沙门氏菌的全基因组进行了比较，并选择了作为ratA基因一部分的1个差异基因组区域作为聚合酶链反应检测的分子标记，以便快速区分这些微生物。总共对26株鸡伤寒沙门氏菌和17株鸡白痢沙门氏菌进行了检测，该检测成功地将它们区分开来。
