Polymer Institute, Slovak Academy of Sciences, Dúbravská cesta 9, 842 36 Bratislava, Slovakia.
Biochem Soc Trans. 2013 Apr;41(2):625-9. doi: 10.1042/BST20120279.
Understanding the mechanism of DNA extension in nanochannels is necessary for interpretation of experiments in nanofluidic channel devices that have been conducted recently with both linear and ring chains. The present article reviews the situation with linear chains and analyses the experimental results and simulations for channel-induced extension (linearization) of ring chains. Results for confined rings indicate a transition between moderate and strong confinement similar to that of linear chains. Owing to stronger self-avoidance in confined rings, the transition and chain extension is shifted relative to linear DNA. We suggest that a relationship similar to that used for the extension of linear chains may also be used for circular DNA.
理解 DNA 在纳米通道中延伸的机制对于解释最近使用线性和环形链在纳流道装置中进行的实验是必要的。本文回顾了线性链的情况，并分析了环链通道诱导延伸（线性化）的实验结果和模拟。受限环的结果表明存在类似于线性链的中等和强受限之间的转变。由于受限环中的自回避更强，因此相对于线性 DNA，转变和链延伸会发生偏移。我们建议，类似于用于线性链延伸的关系也可用于环形 DNA。
