Matoba Satoko, Bender Katrin, Fahey Alan G, Mamo Solomon, Brennan Lorraine, Lonergan Patrick, Fair Trudee
School of Agriculture and Food Science, University College Dublin, Belfield, Dublin 4, Ireland.
Institute of Food and Health, Conway Institute, University College Dublin, Belfield, Dublin 4, Ireland.
Reprod Fertil Dev. 2014 Jan;26(2):337-45. doi: 10.1071/RD13007.
The follicle is a unique micro-environment within which the oocyte can develop and mature to a fertilisable gamete. The aim of this study was to investigate the ability of a panel of follicular parameters, including intrafollicular steroid and metabolomic profiles and theca, granulosa and cumulus cell candidate gene mRNA abundance, to predict the potential of bovine oocytes to develop to the blastocyst stage in vitro. Individual follicles were dissected from abattoir ovaries, carefully ruptured under a stereomicroscope and the oocyte was recovered and individually processed through in vitro maturation, fertilisation and culture. The mean (±s.e.m.) follicular concentrations of testosterone (62.8±4.8 ngmL(-1)), progesterone (616.8±31.9 ngmL(-1)) and oestradiol (14.4±2.4 ngmL(-1)) were not different (P>0.05) between oocytes that formed (competent) or failed to form (incompetent) blastocysts. Principal-component analysis of the quantified aqueous metabolites in follicular fluid showed differences between oocytes that formed blastocysts and oocytes that degenerated; l-alanine, glycine and l-glutamate were positively correlated and urea was negatively correlated with blastocyst formation. Follicular fluid associated with competent oocytes was significantly lower in palmitic acid (P=0.023) and total fatty acids (P=0.031) and significantly higher in linolenic acid (P=0.036) than follicular fluid from incompetent oocytes. Significantly higher (P<0.05) transcript abundance of LHCGR in granulosa cells, ESR1 and VCAN in thecal cells and TNFAIP6 in cumulus cells was associated with competent compared with incompetent oocytes.
卵泡是一个独特的微环境，卵母细胞可在此环境中发育并成熟为可受精的配子。本研究的目的是调查一组卵泡参数的预测能力，这些参数包括卵泡内类固醇和代谢组学特征以及卵泡膜、颗粒细胞和卵丘细胞候选基因的mRNA丰度，以预测牛卵母细胞在体外发育至囊胚阶段的潜力。从屠宰场的卵巢中分离出单个卵泡，在体视显微镜下小心破裂，回收卵母细胞，并分别通过体外成熟、受精和培养进行处理。形成（有能力）或未形成（无能力）囊胚的卵母细胞之间，睾酮（62.8±4.8 ngmL(-1)）、孕酮（616.8±31.9 ngmL(-1)）和雌二醇（14.4±2.4 ngmL(-1)）的平均（±标准误）卵泡浓度无差异（P>0.05）。卵泡液中定量水相代谢物的主成分分析显示，形成囊胚的卵母细胞和退化的卵母细胞之间存在差异；L-丙氨酸、甘氨酸和L-谷氨酸与囊胚形成呈正相关，尿素与囊胚形成呈负相关。与无能力卵母细胞的卵泡液相比，与有能力卵母细胞相关的卵泡液中棕榈酸（P=0.023）和总脂肪酸（P=0.031）含量显著降低，亚麻酸（P=0.036）含量显著升高。与无能力卵母细胞相比，颗粒细胞中LHCGR、卵泡膜细胞中ESR1和VCAN以及卵丘细胞中TNFAIP6的转录本丰度显著更高（P<0.05）与有能力卵母细胞相关。
