Departamento de Bioquímica y Biología Molecular, Facultad de Medicina, Instituto Universitario de Oncología, Universidad de Oviedo, Oviedo, 33006, Spain.
Cell Commun Signal. 2013 Mar 21;11(1):19. doi: 10.1186/1478-811X-11-19.
Monitoring activity of specific signaling pathways in vivo is challenging and requires highly sensitive methods to detect dynamic perturbations in whole organisms.
In vivo gene delivery of a luciferase reporter followed by bioluminiscence imaging allows measuring NF-κB activity in mice liver and lungs.
This protocol allows a direct measure of NF-κB activity through quantification of bioluminescence signal, demonstrating its accuracy and sensitivity in different animal models and experimental conditions. Variants could be also applied for the analysis of NF-κB activity in different tissues or for studying other signaling pathways in vivo.
在体内监测特定信号通路的活性具有挑战性，需要高度敏感的方法来检测整个生物体中动态的扰动。
通过活体基因转染荧光素酶报告基因，再进行生物发光成像，可在小鼠的肝脏和肺部测量 NF-κB 活性。
该方案通过对生物发光信号进行定量来直接测量 NF-κB 活性，证明了其在不同动物模型和实验条件下的准确性和灵敏度。该方法也可用于分析不同组织中的 NF-κB 活性，或用于研究体内其他信号通路。
