[阿比让（科特迪瓦）阴道白色念珠菌对抗真菌药物的体外敏感性]

[In vitro susceptibility of vaginal Candida albicans to antifungal drugs in Abidjan (Ivory Coast)].

作者信息

Djohan V, Angora K E, Vanga-Bosson A H, Konaté A, Kassi F K, Yavo W, Kiki-Barro P C, Menan H, Koné M

机构信息

UFR des sciences pharmaceutiques et biologiques, département de parasitologie-mycologie, université de Cocody (Abidjan), BPV 34, Abidjan, Côte d'Ivoire.

出版信息

J Mycol Med. 2012 Jun;22(2):129-33. doi: 10.1016/j.mycmed.2011.11.005. Epub 2011 Dec 29.

DOI:10.1016/j.mycmed.2011.11.005

PMID:23518014

Abstract

OBJECTIVE

The aim of this study was to evaluate in vitro susceptibility of vaginal Candida albicans to common antifungal drugs in Abidjan, Ivory Coast.

PATIENTS AND METHODS

From January to September 2008, 150 women with leucorrhoea were sampled for vaginal mycosis at the Pasteur Institute (Ivory Coast). Samples were analyzed by direct examination, Sabouraud-chloramphenicol and Sabouraud-chloramphenicol-actidione culture. C. albicans was identified after blastesis, chlamydosporulation and auxanogram tests. The susceptibility of this fungus to amphotericine B, 5-fluorocytosine, fluconazole, itraconazole and voriconazole was evaluated by a semi-solid medium microdilution technique: ATB(®) Fungus 3.

RESULTS

Among 62 yeasts strains isolated, C. albicans represented 45 cases or 72.6%. Vaginal itching (P=0.04) and urinary burning (P=0.002) was statistically correlated with vaginal candidosis. We observed a range of susceptibility of C. albicans strains to antifungals: 100% to amphotericine B (CMI90=0.5μg/mL); 98% to 5-fluorocytosine (CMI90=4μg/mL); 86.7% to voriconazole (CMI50=0.06μg/mL) and 80% to fluconazole (CMI50=2μg/mL and CMI90=32μg/mL). However, only 46.7% of C. albicans strains were sensitive to itraconazole (CMI50=0.125μg/mL).

CONCLUSION

These results show that vaginal C. albicans remain sensitive to the most commonly antifungal drugs used in Abidjan. However, this susceptibility should be regularly monitored.

摘要

目的

本研究旨在评估科特迪瓦阿比让阴道白色念珠菌对常见抗真菌药物的体外敏感性。

患者与方法

2008年1月至9月，在科特迪瓦巴斯德研究所对150名白带异常的女性进行阴道真菌病采样。样本通过直接检查、沙氏氯霉素培养基和沙氏氯霉素放线菌酮培养基培养进行分析。白色念珠菌经芽殖、厚垣孢子形成和营养谱试验鉴定。采用半固体培养基微量稀释技术（ATB(®) Fungus 3）评估该真菌对两性霉素B、5-氟胞嘧啶、氟康唑、伊曲康唑和伏立康唑的敏感性。

结果

在分离出的62株酵母菌株中，白色念珠菌占45例，即72.6%。阴道瘙痒（P = 0.04）和尿痛（P = 0.002）与阴道念珠菌病存在统计学相关性。我们观察到白色念珠菌菌株对不同抗真菌药物的敏感性范围：对两性霉素B的敏感性为100%（CMI90 = 0.5μg/mL）；对5-氟胞嘧啶为98%（CMI90 = 4μg/mL）；对伏立康唑为86.7%（CMI50 = 0.06μg/mL）；对氟康唑为80%（CMI50 = 2μg/mL，CMI90 = 32μg/mL）。然而，只有46.7%的白色念珠菌菌株对伊曲康唑敏感（CMI50 = 0.125μg/mL）。