通过改变聚丙烯酰胺凝胶电泳条件有效分离52 kDa SSA/Ro多肽与48 kDa SSB/La多肽。
Effective separation of the 52 kDa SSA/Ro polypeptide from the 48 kDa SSB/La polypeptide by altering conditions of polyacrylamide gel electrophoresis.
作者信息
Buyon J P, Slade S G, Chan E K, Tan E M, Winchester R
机构信息
Institute of Molecular Immunology, Hospital for Joint Diseases of the New York University Medical Center, New York 10003.
出版信息
J Immunol Methods. 1990 May 25;129(2):207-10. doi: 10.1016/0022-1759(90)90440-7.
A method is described for the separation of the 52 kDa SSA/Ro polypeptide from the 48 kDa SSB/La polypeptide. These two proteins anomalously co-migrate with the same relative mass under conditions of conventional sodium dodecyl sulfate polyacrylamide gel electrophoresis according to Laemmli using a stock solution of acrylamide: bisacrylamide 30:0.8, ratio = 37.5. A higher ratio of monomer to cross-linker, ratio = 172.4 used in a 15% acrylamide solution, readily separates the two peptide chains. This method facilitates the detection of the 52 kDa SSA/Ro component which otherwise might have been incorrectly assigned as a 48 kDa SSB/La polypeptide.
本文描述了一种从48 kDa SSB/La多肽中分离52 kDa SSA/Ro多肽的方法。在使用丙烯酰胺：双丙烯酰胺30:0.8（比例 = 37.5）的储备溶液按照Laemmli方法进行的传统十二烷基硫酸钠聚丙烯酰胺凝胶电泳条件下，这两种蛋白质会以相同的相对分子量异常共迁移。在15%丙烯酰胺溶液中使用更高的单体与交联剂比例（比例 = 172.4），可以轻松分离这两条肽链。该方法有助于检测52 kDa SSA/Ro组分，否则它可能会被错误地认定为48 kDa SSB/La多肽。
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