Jiao Qian, Xie Wu-ling, Wang Yuan-yuan, Chen Xin-lin, Yang Peng-bo, Zhang Peng-bo, Tan Jing, Lu Hai-xia, Liu Yong
Institute of Neurobiology, Xi'an Jiaotong University College of Medicine, Xi'an, Shaanxi, 710061, PR China.
Int J Dev Neurosci. 2013 Jun;31(4):280-5. doi: 10.1016/j.ijdevneu.2013.03.007. Epub 2013 Mar 18.
Neurogenesis and angiogenesis are two parallel processes that occur in brain development and repair, and so share some molecular signals. In order to better understand the interaction between the genesis of neural cells and vessels during brain development, the density of microvessels and the number of nestin positive neural stem/neural progenitor cells (NSCs/NPCs) around microvasculature in various brain regions was quantified. Results showed that the density of microvessels remained at a relative low level during embryonic development and dramatically increased after postnatal day 3 (P3), especially in subventricular zone. The number of nestin positive NSCs/NPCs per microvessel in neurogenic brain regions continually increased with fetal brain development and then gradually dropped down during postnatal development. The highest density of NSCs/NPCs appeared at postnatal day 1 (P1) and dramatically decreased after P3. Similar pattern was observed in striatum. In the olfactory bulb, the cerebral cortex and cerebellum, the dramatic decrease of NSCs/NPCs density appeared after P7, especially in the cerebral cortex. Our results demonstrated that anatomically, the spatial relationship between NSCs/NPCs and microvessels changed during brain development. The alteration patterns in neurogenic brain regions differed from non-neurogenic brain regions.
神经发生和血管生成是在大脑发育和修复过程中发生的两个并行过程，因此共享一些分子信号。为了更好地理解大脑发育过程中神经细胞和血管生成之间的相互作用，对不同脑区微血管周围的微血管密度以及巢蛋白阳性神经干细胞/神经祖细胞（NSCs/NPCs）的数量进行了量化。结果表明，微血管密度在胚胎发育期间保持在相对较低的水平，在出生后第3天（P3）后显著增加，尤其是在脑室下区。神经源性脑区中每个微血管的巢蛋白阳性NSCs/NPCs数量随着胎儿大脑发育持续增加，然后在出生后发育过程中逐渐下降。NSCs/NPCs的最高密度出现在出生后第1天（P1），并在P3后显著下降。在纹状体中也观察到类似的模式。在嗅球、大脑皮层和小脑中，NSCs/NPCs密度在P7后显著下降，尤其是在大脑皮层。我们的结果表明，在解剖学上，NSCs/NPCs与微血管之间的空间关系在大脑发育过程中发生了变化。神经源性脑区的变化模式与非神经源性脑区不同。
