Papadopoulos Georgios, Vlachodimitropoulos Dimitrios, Kyroudi Aspasia, Kouloukoussa Mirsini, Perrea Despina, Mitropoulos Dionisios
Department of Histology and Embryology, University of Athens Medical School, Greece.
J Clin Med Res. 2013 Apr;5(2):127-31. doi: 10.4021/jocmr1215w. Epub 2013 Feb 25.
Quinazoline-based alpha1-adrenergic receptor antagonists may not act solely on smooth muscle contractility. We evaluated the in vivo effect of terazosin on the expression of caspase-3 in the rat ventral prostate.
Fifteen Wistar rats were treated with terazosin (1.2 mg/kg body weight, given orally every second day) for 120 days. Another 15 control animals received the same amount of distilled water. The expression of caspase-3 was assessed immunohistochemically in formalin-fixed, paraffin-embedded tissue sections.
Terazosin treatment did not affect prostate weight and histomorphology. In controls caspase-3 was expressed weakly and sporadically. In contrast, strong and weak expression was evident in 67% and 33% of the terazosin-treated specimens, respectively.
These findings implicate the induction of caspase-3 expression by terazosin as a potential molecular mechanism of its apoptotic action on prostate cells.
基于喹唑啉的α1-肾上腺素能受体拮抗剂可能并非仅作用于平滑肌收缩性。我们评估了特拉唑嗪对大鼠腹侧前列腺中半胱天冬酶-3表达的体内效应。
15只Wistar大鼠接受特拉唑嗪治疗（1.2毫克/千克体重，每隔一天口服给药），持续120天。另外15只对照动物接受等量蒸馏水。在福尔马林固定、石蜡包埋的组织切片中通过免疫组织化学法评估半胱天冬酶-3的表达。
特拉唑嗪治疗不影响前列腺重量和组织形态学。在对照中，半胱天冬酶-3表达微弱且散在。相比之下，在接受特拉唑嗪治疗的标本中，分别有67%和33%呈现强表达和弱表达。
这些发现表明特拉唑嗪诱导半胱天冬酶-3表达是其对前列腺细胞凋亡作用的潜在分子机制。
