EPR 光谱学研究银屑病指甲。
EPR spectroscopic investigation of psoriatic finger nails.
机构信息
Department of Radiological Life Sciences, Graduate School of Health Sciences, Hirosaki University, 66-1 Hon-cyo, Hirosaki, 036-8564, Japan.
出版信息
Skin Res Technol. 2013 Nov;19(4):450-3. doi: 10.1111/srt.12068. Epub 2013 Mar 22.
BACKGROUND
Nail lesions are common features of psoriasis and found in almost half of the patients. However, there is no feasible spectroscopic method evaluating changes and severity of nail psoriasis. EPR (electron paramagnetic resonance) might be feasible for evaluating nail conditions in the patients of psoriasis.
METHODS
Finger nails of five cases with nail psoriasis, (three females and two males) were examined. Nail samples were subjected to the EPR assay. The small piece of the finger nail (1.5 × 5 mm(2)) was incubated in ~50 μM 5-DSA (5-doxylstearic acid) aqueous solutions for about 60 min at 37°C. After rinsing and wiping off the excess 5-DSA solution, the nail samples were measured by EPR.
RESULTS
EPR spectra were analyzed using the intensity ratio (Fast/Slow) of the two motions at the peaks of the lower magnetic field. We observed two distinguishable sites on the basis of the EPR results. In addition, the modern EPR calculation was performed to analyze the spectra obtained. The nail psoriasis-related region is 2~3 times higher than that of the control.
CONCLUSION
The present EPR results show that there are two distinguishable sites in the nail. In the case of nail psoriasis, the fragile components are 2~3 times more than those of the control. Thus, the EPR method is thought to be a novel and reliable method of evaluating the nail psoriasis.
背景
指甲病变是银屑病的常见特征，几乎半数患者存在指甲病变。然而，目前尚无可用于评估指甲银屑病变化和严重程度的可行光谱方法。电子顺磁共振（EPR）可能可用于评估银屑病患者的指甲状况。
方法
对 5 例指甲银屑病患者（3 名女性和 2 名男性）的指甲进行了检查。对指甲样本进行 EPR 检测。将 1.5×5mm（2）的小块指甲置于约 50μM 5-DSA（5-二氧辛基琥珀酸）水溶液中，在 37°C 下孵育约 60 分钟。冲洗并擦去多余的 5-DSA 溶液后，通过 EPR 测量指甲样本。
结果
使用磁场下较低峰处两个运动的强度比（Fast/Slow）分析 EPR 光谱。根据 EPR 结果，我们观察到两个可区分的部位。此外，还对获得的光谱进行了现代 EPR 计算分析。指甲银屑病相关区域的强度比对照高 2~3 倍。
结论
目前的 EPR 结果表明指甲中有两个可区分的部位。在指甲银屑病的情况下，易碎成分比对照高 2~3 倍。因此，EPR 方法被认为是评估指甲银屑病的一种新颖且可靠的方法。
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