Li Chao-wang, Zhao Jin-ming, Zhang Chuan-shan, Lv Guo-dong, Wen Hao, Lin Ren-yong
Department of Breast Surgery and Pediatric Surgery, First Affiliated Hospital of Xinjiang Medical University, Urumqi, China.
Zhonghua Gan Zang Bing Za Zhi. 2012 Dec;20(12):930-4. doi: 10.3760/cma.j.issn.1007-3418.2012.12.012.
To investigate whether Echinococcus granulosus cyst fluid-infected host liver cells had differential expression of mitogen-activated protein kinases (MAPKs) or differential cell cycle activity.
Human liver cells cultured with different concentrations of hydatid cyst fluid (HCF) were tested by the MTT method to determine effects on proliferation. The cell cycle was assessed by flow cytometry. Western blotting was used to detect changes in protein expressions of p-ERK, PCNA, cyclin-A, cyclin-B1, cyclin-D1, and cyclin-E.
Forty-eight, 72 and 96 h of HCF at 15%, 30% and 60% concentrations in the cell media significantly promoted cell proliferation (F=67.845, P less than 0.01) and compared to controls (P less than 0.05). Cells exposed to 15% HCF for 48 h showed significantly induced expression of p-ERK (F=1.916, P less than 0.01), higher than controls (P less than 0.01). Cells exposed to 15% HCF for 24 h showed significantly induced expression of cyclin-Dl (F=3.901, P less than 0.01), higher than controls (P less than 0.01). Cells exposed to 15% HCF for 48 h or 30% HCF for 72 h showed significantly induced expression of PCNA (F=91.140, P less than 0.01), higher than controls (P less than 0.01). Cells exposed to 15% HCF for 48 h or 30% HCF for 72 h shed significantly induced expression of cyclin-A (F=18.587, P=0.002), higher than controls (P less than 0.01). Cells exposed to 15% HCF for either 48 h or 72 h showed significantly induced expression of cyclin-B1 (F=2.064, P less than 0.01), higher than controls (P less than 0.01). Cells exposed to 30% HCF for 96 h showed significantly induced expression of cyclin-E (F=1.068, P less than 0.01), higher than controls (P less than 0.01).
Hydatid cyst fluid exerts no inhibitory effect on primary cultured host liver cells, but may promote cellular proliferation.
研究细粒棘球绦虫囊液感染宿主肝细胞后丝裂原活化蛋白激酶（MAPKs）表达是否存在差异或细胞周期活性是否存在差异。
用不同浓度的包虫囊液（HCF）培养人肝细胞，采用MTT法检测其对细胞增殖的影响。通过流式细胞术评估细胞周期。采用蛋白质免疫印迹法检测p-ERK、PCNA、细胞周期蛋白A、细胞周期蛋白B1、细胞周期蛋白D1和细胞周期蛋白E的蛋白表达变化。
细胞培养基中15%、30%和60%浓度的HCF作用48、72和96小时均显著促进细胞增殖（F=67.845，P<0.01），与对照组相比差异有统计学意义（P<0.05）。15% HCF作用48小时的细胞p-ERK表达显著上调（F=1.916，P<0.01），高于对照组（P<0.01）。15% HCF作用24小时的细胞细胞周期蛋白D1表达显著上调（F=3.901，P<0.01），高于对照组（P<0.01）。15% HCF作用48小时或30% HCF作用72小时的细胞PCNA表达显著上调（F=91.140，P<0.01），高于对照组（P<0.01）。15% HCF作用48小时或30% HCF作用72小时的细胞细胞周期蛋白A表达显著上调（F=18.587，P=0.002），高于对照组（P<0.01）。15% HCF作用48小时或72小时的细胞细胞周期蛋白B1表达显著上调（F=2.064，P<0.01），高于对照组（P<0.01）。30% HCF作用96小时的细胞细胞周期蛋白E表达显著上调（F=1.068，P<0.01），高于对照组（P<0.01）。
包虫囊液对原代培养的宿主肝细胞无抑制作用，反而可能促进细胞增殖。
