Vocational School of Health Services, Hacettepe University, 06100, Ankara, Turkey.
Vocational School of Health Services, Yüksek İhtisas University, 06291, Ankara, Turkey.
Acta Parasitol. 2024 Mar;69(1):700-709. doi: 10.1007/s11686-024-00797-z. Epub 2024 Feb 19.
Cystic Echinococcosis (CE) is a zoonotic infection caused by the larval form of Echinococcus granulosus in humans. Emerging evidence suggests an intriguing inverse association between E. granulosus infection and the occurrence of cancer. This study aimed to investigate the influence of diverse host-derived hydatid cyst fluids (HCF) with distinct genotypes on human liver hepatocytes (HC) and hepatocellular carcinoma cells (HepG2). Specifically, we examined their effects on cell proliferation, apoptosis sensitivity (BAX/BCL-2), apoptosis-related p53 expression, and the expression of cancer-related microRNA (hsa-miR-181b-3p). Cell proliferation assays, real-time PCR, and ELISA studies were conducted to evaluate potential anti-cancer properties. The findings revealed that animal-origin HCF (G1(A)) induced direct cell death by augmenting the susceptibility of HepG2 cells to apoptosis. Treatment with both G1(A) and G1(H) HCF sensitized HepG2 and HC cell lines to apoptosis by modulating the BAX/BCL-2 ratio, accompanied by upregulation of the p53 gene. Additionally, G1(A) HCF and human-derived HCFs (G1(H), G7(H)) reduced the expression of miR-181b-3p in HepG2 cells. Consequently, this study demonstrates the potential anti-cancer effect of HCF in HepG2 cells and provides the first comparative assessment of HCFs from human and animal sources with diverse genotypes, offering novel insights into this field.
泡型包虫病（CE）是一种由细粒棘球绦虫幼虫引起的人畜共患感染。新出现的证据表明，细粒棘球蚴感染与癌症的发生之间存在有趣的负相关关系。本研究旨在研究不同宿主来源的具有不同基因型的包虫囊液（HCF）对人肝实质细胞（HC）和肝癌细胞（HepG2）的影响。具体来说，我们研究了它们对细胞增殖、凋亡敏感性（BAX/BCL-2）、凋亡相关 p53 表达和癌症相关 microRNA（hsa-miR-181b-3p）表达的影响。通过细胞增殖测定、实时 PCR 和 ELISA 研究来评估潜在的抗癌特性。研究结果表明，动物源 HCF（G1(A)）通过增加 HepG2 细胞对凋亡的敏感性来诱导直接细胞死亡。用 G1(A)和 G1(H) HCF 处理 HepG2 和 HC 细胞系，通过调节 BAX/BCL-2 比值，同时上调 p53 基因，使 HepG2 和 HC 细胞系对凋亡敏感。此外，G1(A) HCF 和人源 HCF（G1(H)、G7(H)）降低了 HepG2 细胞中 miR-181b-3p 的表达。因此，本研究证明了 HCF 在 HepG2 细胞中的潜在抗癌作用，并首次对具有不同基因型的人源和动物源 HCF 进行了比较评估，为该领域提供了新的见解。
