Institut de Chimie de Nice, Université de Nice-Sophia Antipolis, UMR 7272 CNRS, Parc Valrose, 06108 Nice Cedex 2, France.
J Chromatogr A. 2013 May 3;1288:127-48. doi: 10.1016/j.chroma.2013.03.002. Epub 2013 Mar 13.
Vetiver essential oils (VEO) are important raw ingredients used in perfume industry, entering the formula of numerous modern fragrances. Vetiver oils are considered to be among the most complex essential oils, resulting most of the time in highly coeluted chromatograms whatever the analytical technique. In this context, conventional gas chromatography has failed to provide a routine tool for the accurate qualitative and quantitative analysis of their constituents. Applying comprehensive two-dimensional gas chromatography techniques (GC×GC-FID/MS) afforded the mean to separate efficiently vetiver oil constituents in order to identify them in a more reliable way. Moreover, this is the first time that a complete true quantitation of each constituent is carried out on such complex oils by means of internal calibration. Finally, we have studied the influence of the injection mode on the determined chemical composition, and showed that several alcohols underwent dehydration under defined chromatographic conditions (splitless mode) usually recommended for quantitation purposes.
香根草精油（VEO）是香水行业的重要原料，许多现代香水都采用了香根草精油。香根草精油被认为是最复杂的精油之一，无论采用何种分析技术，其色谱图往往高度共洗脱。在这种情况下，常规气相色谱未能为其成分的准确定性和定量分析提供常规工具。应用全二维气相色谱技术（GC×GC-FID/MS）可以有效地分离香根草油成分，从而更可靠地对其进行鉴定。此外，这是第一次通过内部校准对如此复杂的油进行每个成分的完整真正定量。最后，我们研究了进样模式对所确定化学成分的影响，并表明在通常推荐用于定量目的的规定色谱条件（不分流模式）下，几种醇发生了脱水。
