Singh I N, Sorrentino G, McCartney D G, Massarelli R, Kanfer J N
Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Manitoba, Winnipeg, Canada.
J Neurosci Res. 1990 Apr;25(4):476-85. doi: 10.1002/jnr.490250405.
The presence of 10(-5) M retinoic acid (RA) in the culture medium of LA-N-1, a catecholaminergic cell line, and LA-N-2, a cholinergic cell line, enhanced their morphological differentiation. Tyrosine hydroxylase (TH) activity of the LA-N-1 cells was increased in the RA-treated cells compared with control cultures at day 4 and remained elevated. Choline acetyltransferase (ChAT) activity in the LA-N-2 cells gradually increased until 8 days in vitro (DIV) both in the untreated control and the RA treated cultures. This activity in control and treated cells decreased gradually to a constant level of activity. The ChAT activity at 8 DIV of RA-treated LA-N-2 cells was increased 2.1-fold (P less than 0.001) as compared to the control cultures. This increase in ChAT activity was accompanied by a 73% decrease of acetylcholinesterase (AChE) activity in LA-N-2 cells by 8 DIV. AChE activity of LA-N-1 cells was unchanged during the time course of the experiment. Phospholipase-A2 (PL-A2) activity in RA-treated LA-N-2 cells was increased at day 4 as compared with the control cultures. There were no differences observed in phospholipase-D (PL-D), choline kinase and GPC-phosphodiesterases activities in RA-treated and -untreated LA-N-1 and LA-N-2 cells.
在儿茶酚胺能细胞系LA-N-1和胆碱能细胞系LA-N-2的培养基中加入10(-5) M的视黄酸（RA），可增强它们的形态分化。与对照培养物相比，在第4天，RA处理的LA-N-1细胞的酪氨酸羟化酶（TH）活性增加，并持续升高。在未处理的对照培养物和RA处理的培养物中，LA-N-2细胞中的胆碱乙酰转移酶（ChAT）活性在体外培养8天（DIV）之前逐渐增加。对照细胞和处理细胞中的这种活性逐渐下降至恒定水平。与对照培养物相比，RA处理的LA-N-2细胞在8 DIV时的ChAT活性增加了2.1倍（P < 0.001）。到8 DIV时，LA-N-2细胞中ChAT活性的增加伴随着乙酰胆碱酯酶（AChE）活性下降73%。在实验过程中，LA-N-1细胞的AChE活性没有变化。与对照培养物相比，RA处理的LA-N-2细胞在第4天的磷脂酶A2（PL-A2）活性增加。在RA处理和未处理的LA-N-1和LA-N-2细胞中，未观察到磷脂酶D（PL-D）、胆碱激酶和甘油磷酸胆碱磷酸二酯酶活性的差异。
