Avian Disease Laboratory, College of Veterinary Medicine, Konkuk University, Seoul 143-701, Republic of Korea.
Antiviral Res. 2013 May;98(2):284-90. doi: 10.1016/j.antiviral.2013.03.013. Epub 2013 Mar 21.
The extensive morbidity and mortality caused by influenza A viruses worldwide prompts the need for a deeper understanding of the host immune response and novel therapeutic and/or prophylactic interventions. In this study, we assessed the sublingual route as an effective means of delivering probiotics against influenza virus in mice. In addition, IgA levels, NK cell activity, T cell activation, and cytokine profiles in the lungs were examined to understand the mechanism underlying this protective effect. Sublingual administration of Lactobacillus rhamnosus provided enhanced protection against influenza virus infection by enhancing mucosal secretory IgA production, and T and NK cell activity. Moreover, interleukin (IL)-12 levels in the lungs increased significantly. Conversely, IL-6 and tumor necrosis factor alpha levels in the lungs decreased significantly. On the basis of these promising findings, we propose that the sublingual mucosal route is an attractive alternative to mucosal routes for administering probiotics against influenza virus.
甲型流感病毒在全球范围内造成了广泛的发病率和死亡率，这促使人们需要更深入地了解宿主免疫反应以及新的治疗和/或预防干预措施。在这项研究中，我们评估了舌下途径作为向小鼠递送抗流感病毒益生菌的有效手段。此外，还检查了肺部的 IgA 水平、NK 细胞活性、T 细胞激活和细胞因子谱，以了解这种保护作用的机制。鼠李糖乳杆菌的舌下给药通过增强黏膜分泌型 IgA 的产生以及 T 和 NK 细胞的活性，提供了对流感病毒感染的增强保护。此外，肺部的白细胞介素（IL）-12 水平显著增加。相反，肺部的 IL-6 和肿瘤坏死因子 alpha 水平显著降低。基于这些有希望的发现，我们提出舌下黏膜途径是向黏膜途径施用抗流感病毒益生菌的一种有吸引力的替代方法。
