Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR China.
Biochem Biophys Res Commun. 2013 Apr 19;433(4):463-9. doi: 10.1016/j.bbrc.2013.03.038. Epub 2013 Mar 22.
Chemoreception is a key feature in selection of host plants by insects. In this study, preliminary characterization and isolation of cDNA clones from Cnaphalocrocis medinalis antennal libraries identified eight olfactory genes, including two putative general odorant-binding proteins (GOBPs), three pheromone binding proteins (PBPs) and three chemosensory proteins (CSPs). The expression profiles of these eight genes in different tissues (antenna, head (without antennae), thorax, abdomen, leg and wing) were measured by real time qPCR. The results showed that GOBP and PBP genes in C. medinalis seemed to be antenna-specific, but differentially expressed in male and female antennae; while CSP genes were expressed ubiquitously during different developmental stages, but with an extremely elevated transcript level in antennae, legs and wings compared to head, thorax and abdomen. And also, the transcription levels of olfactory genes depended on the age, sex, and mating status of the adults. These findings support the hypothesis that OBPs and CSPs play dynamic roles during development of C. medinalis and are likely to be involved in broader physiological functions.
化感作用是昆虫选择寄主植物的一个关键特征。在这项研究中，初步鉴定和分离了小菜蛾触角文库中的 cDNA 克隆，鉴定出了 8 个嗅觉基因，包括 2 个假定的通用气味结合蛋白（GOBP）、3 个信息素结合蛋白（PBP）和 3 个化学感受蛋白（CSP）。通过实时 qPCR 测量了这 8 个基因在不同组织（触角、头部（无触角）、胸部、腹部、腿部和翅膀）中的表达谱。结果表明，小菜蛾的 GOBP 和 PBP 基因似乎是触角特异性的，但在雌雄触角中表达不同；而 CSP 基因在不同发育阶段普遍表达，但在触角、腿部和翅膀中的转录水平极高，与头部、胸部和腹部相比。此外，嗅觉基因的转录水平取决于成虫的年龄、性别和交配状态。这些发现支持了 OBPs 和 CSPs 在小菜蛾发育过程中发挥动态作用的假设，并且可能涉及更广泛的生理功能。
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