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用于质谱检测的液相色谱衍生化

Derivatization in liquid chromatography for mass spectrometric detection.

作者信息

Santa T

机构信息

Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo, Japan.

出版信息

Drug Discov Ther. 2013 Feb;7(1):9-17. doi: 10.5582/ddt.2013.v7.1.9.

DOI:10.5582/ddt.2013.v7.1.9
PMID:23524938
Abstract

Liquid chromatography coupled with electrospray ionization tandem mass spectrometry (LC/ESI-MS/MS) has been frequently utilized for the sensitive and selective determination of the trace level compounds in biological samples. In LC/ESI-MS/MS, chemical derivatization is sometimes used to enhance the detection sensitivity of the analytes. This review presents an overview of the derivatization reagents in LC/ESI-MS/MS that have been applied to the low molecular weight compounds in recent five years (2008-2012).

摘要

液相色谱联用电喷雾电离串联质谱法（LC/ESI-MS/MS）已被频繁用于生物样品中痕量化合物的灵敏且选择性测定。在LC/ESI-MS/MS中，有时会使用化学衍生化来提高分析物的检测灵敏度。本综述概述了近五年（2008 - 2012年）在LC/ESI-MS/MS中应用于低分子量化合物的衍生化试剂。

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