Oo Thandar, Sasaki Noboru, Ikenaka Yoshinori, Ichise Takahiro, Nagata Noriyuki, Yokoyama Nozomu, Sasaoka Kazuyoshi, Morishita Keitaro, Nakamura Kensuke, Takiguchi Mitsuyoshi
Laboratory of Veterinary Internal Medicine, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
Veterinary Teaching Hospital, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
Front Vet Sci. 2022 Sep 28;9:1014792. doi: 10.3389/fvets.2022.1014792. eCollection 2022.
Hepatocellular carcinoma (HCC) is one of the most common primary liver tumors in humans and dogs. Excessive adrenocortical hormone exposure may cause steroid hepatopathy, which may develop into HCC. In our previous study, hyperadrenocorticism (HAC) was a highly concurrent disease in dogs with HCC. Therefore, this study hypothesized that adrenal steroid alterations might be involved in hepatocarcinogenesis and aimed to specify the relationship between HAC and HCC in dogs.
This study included 46 dogs brought to the Hokkaido University Veterinary Teaching Hospital between March 2019 and December 2020. Owners gave their signed consent for blood collection on their first visit. A total of 19 steroids (14 steroids and 5 metabolites) in the baseline serum of 15 dogs with HCC, 15 dogs with HAC, and 10 dogs with both diseases were quantitatively measured using the developed liquid chromatography coupled with tandem mass spectrometry (LC/MS/MS) method.
In each group, 11 steroids were detected higher than 50%. The detection rate of steroid hormones did not significantly differ between the groups ( > 0.05). Principle component analysis (PCA) showed that the steroid profiles of the three groups were comparable. Median steroid hormone concentrations were not significantly different between the study diseases ( > 0.05).
The developed LC/MS/MS was useful for measuring steroid hormones. Although it was clear that HAC was concurrent in dogs with HCC, none of the serum steroids was suggested to be involved in HCC.
肝细胞癌（HCC）是人类和犬类中最常见的原发性肝脏肿瘤之一。肾上腺皮质激素暴露过多可能导致类固醇性肝病，进而可能发展为HCC。在我们之前的研究中，肾上腺皮质功能亢进（HAC）是患有HCC的犬类中一种高度并发的疾病。因此，本研究假设肾上腺类固醇改变可能参与肝癌发生，并旨在明确犬类中HAC与HCC之间的关系。
本研究纳入了2019年3月至2020年12月期间送至北海道大学兽医教学医院的46只犬。主人在首次就诊时签署了同意采血的知情同意书。使用开发的液相色谱-串联质谱（LC/MS/MS）方法对15只患有HCC的犬、15只患有HAC的犬和10只同时患有这两种疾病的犬的基线血清中的总共19种类固醇（14种类固醇和5种代谢物）进行了定量测定。
在每组中，检测到11种类固醇的比例高于50%。各组之间类固醇激素的检测率没有显著差异（>0.05）。主成分分析（PCA）表明三组的类固醇谱具有可比性。研究疾病之间的类固醇激素中位数浓度没有显著差异（>0.05）。
开发的LC/MS/MS方法可用于测量类固醇激素。虽然很明显HAC在患有HCC的犬类中并发，但没有血清类固醇被认为与HCC有关。
