Kikuta K, Kurata H, Nagamine T, Aizawa C, Ueno Y, Kurata T, Tamura S
Department of Pathology, National Institute of Health, Tokyo.
Microbiol Immunol. 1990;34(3):337-46. doi: 10.1111/j.1348-0421.1990.tb01014.x.
The effects of B subunit of cholera toxin (CTB) on delayed-type hypersensitivity (DTH) response to influenza vaccine derived from influenza virus A/PR/8/34 (PR-8, HlNl) virus were investigated in B10 mice that were immunized intranasally with both influenza vaccine and CTB. The result showed that intranasal inoculation of this combination augmented DTH response to influenza vaccine, which reached its peak 6 days after inoculation, and also induced accelerated DTH response upon a second inoculation of influenza vaccine alone 4 weeks later, that the cross-reactive DTH response to PR-8 vaccine was elicited by the injection of the different influenza A-type virus vaccine into the footpad of the vaccinated mice, but was not by influenza B-type virus vaccine, that the DTH-mediating T cells were detected selectively in the lungs of mice that received the nasal inoculation of the vaccine and CTB together, but that subcutaneous inoculation of this combination failed to induce DTH-mediating T cells in the lungs. These results, together with the previous papers (Tamura et al, Vaccine 7: 257-262; 314-320, 1989), suggest that CTB could augment both humoral and DTH responses against influenza vaccine in the respiratory mucosal tract.
研究了霍乱毒素B亚基（CTB）对B10小鼠经鼻接种流感疫苗（源自甲型流感病毒A/PR/8/34，PR - 8，H1N1）后对流感疫苗迟发型超敏反应（DTH）的影响。结果显示，经鼻接种该组合可增强对流感疫苗的DTH反应，接种后6天达到峰值，并且在4周后再次单独接种流感疫苗时还可诱导加速的DTH反应；向接种疫苗的小鼠足垫注射不同的甲型流感病毒疫苗可引发对PR - 8疫苗的交叉反应性DTH反应，但注射乙型流感病毒疫苗则不会引发；在同时经鼻接种疫苗和CTB的小鼠肺中选择性检测到了介导DTH的T细胞，但皮下接种该组合未能在肺中诱导介导DTH的T细胞。这些结果与之前的论文（Tamura等人，《疫苗》7：257 - 262；314 - 320，1989）一起表明，CTB可增强呼吸道黏膜对流感疫苗的体液免疫和DTH反应。
