Clin Chem Lab Med. 2014 Jan 1;52(1):129-38. doi: 10.1515/cclm-2012-0850.
High levels of circulating advanced glycation end products (AGEs) can initiate chronic low-grade activation of the immune system (CLAIS) with each of these factors independently associated with cardiovascular (CV) morbidity and mortality. Therefore, our objective was to characterize the relationship between serum AGEs, CLAIS and other risk factors for CV disease in normotensive non-diabetic individuals.
We measured body mass index (BMI), waist-to-hip ratio (WHR), blood pressure, lipid and glucose profile in 44 non-diabetic volunteers (17 female, 27 males). Carboxymethyl-lysine (CML) was measured by ELISA as a marker for circulating AGEs and NF-κB p65 activity as an inflammatory marker by DNA-binding in peripheral blood mononuclear cells lysates (PBMC).
Plasma CML concentrations were related to diastolic blood pressure (r=-0.51, p<0.01) independently of age, sex, BMI and WHR (p<0.05). Diastolic blood pressure was also related to NF-κB activity in PBMC (r=0.47, p<0.01) before and after adjustment for age, sex, BMI and WHR (p<0.05). Plasma CML concentrations were related to the pulse pressure before (r=0.42; p<0.05) and after adjustment for age, sex, BMI and waist (p<0.05). Neither CML nor NF-κB activity were related to systolic blood pressure (both p=ns). Plasma CML concentrations were not associated with plasma lipid or glucose concentrations (all p=ns).
Plasma AGE levels and NF-κB activity in PBMC were independent determinants of diastolic and pulse pressure in healthy normotensive individuals. This association suggests a role for AGEs in the etiology of hypertension, possibly via the initiation of CLAIS and aortic stiffening.
高水平的循环晚期糖基化终产物（AGEs）可引发慢性低度免疫激活（CLAIS），这些因素均与心血管（CV）发病率和死亡率独立相关。因此，我们的目的是描述在血压正常的非糖尿病个体中，血清 AGEs、CLAIS 与其他 CV 疾病危险因素之间的关系。
我们测量了 44 名非糖尿病志愿者（17 名女性，27 名男性）的体重指数（BMI）、腰臀比（WHR）、血压、血脂和血糖谱。通过 ELISA 测量羧甲基赖氨酸（CML）作为循环 AGEs 的标志物，通过外周血单核细胞裂解物（PBMC）中的 DNA 结合来测量 NF-κB p65 活性作为炎症标志物。
血浆 CML 浓度与舒张压（r=-0.51，p<0.01）独立相关，与年龄、性别、BMI 和 WHR 无关（p<0.05）。在调整年龄、性别、BMI 和 WHR 后，舒张压也与 PBMC 中的 NF-κB 活性相关（r=0.47，p<0.01）（p<0.05）。血浆 CML 浓度与调整年龄、性别、BMI 和腰围前后的脉压呈正相关（r=0.42；p<0.05）。CML 和 NF-κB 活性均与收缩压无关（均 p=ns）。血浆 CML 浓度与血浆脂质或血糖浓度无关（均 p=ns）。
血浆 AGE 水平和 PBMC 中的 NF-κB 活性是健康血压正常个体舒张压和脉压的独立决定因素。这种相关性表明 AGEs 在高血压的发病机制中起作用，可能通过 CLAIS 的启动和主动脉僵硬。
