Key Lab of Integrated Crop Pest Management of Shandong Province, College of Agronomy and Plant Protection, Qingdao Agricultural University, Qingdao, Shandong Province, China.
PLoS One. 2013;8(3):e59884. doi: 10.1371/journal.pone.0059884. Epub 2013 Mar 20.
MicroRNAs (miRNAs) are small, conserved, non-coding RNAs that post-transcriptionally regulate gene expression. Bemisia tabaci (Gennadius) B and Q are two invasive and dominant whiteflies, and B. tabaci Q has been displacing B in China. Differences in biological traits (fecundity, host range, resistance to insecticides, etc.) as affected by miRNAs might be involved in the displacement. In this study, we performed high-throughput sequencing to identify miRNAs in B. tabaci B and Q.
We identified 170 conserved miRNAs and 15 novel candidates, and found significant differences in the expression of miRNAs between B. tabaci B and Q.
Expression levels of miRNAs differ in B. tabaci B vs. Q. Additional research is needed to determine whether these differences are related to differences in the biology of B. tabaci B and Q, and whether these differences help explain why B. tabaci Q is displacing B in China.
MicroRNAs (miRNAs) 是小的、保守的、非编码的 RNA，可在后转录水平上调节基因表达。烟粉虱（Bemisia tabaci）B 和 Q 是两种入侵性和优势性的粉虱，B. tabaci Q 已在中国取代 B。miRNAs 可能影响生物特征（繁殖力、宿主范围、抗药性等）的差异，可能涉及到这种取代。在这项研究中，我们进行了高通量测序以鉴定 B. tabaci B 和 Q 中的 miRNAs。
我们鉴定出 170 个保守的 miRNAs 和 15 个新的候选 miRNA，发现 B. tabaci B 和 Q 之间 miRNA 的表达存在显著差异。
B. tabaci B 和 Q 之间的 miRNAs 表达水平存在差异。需要进一步研究以确定这些差异是否与 B. tabaci B 和 Q 的生物学差异有关，以及这些差异是否有助于解释为什么 B. tabaci Q 在中国取代了 B。
