Bubici Giovanni, Prigigallo Maria Isabella, Garganese Francesca, Nugnes Francesco, Jansen Maurice, Porcelli Francesco
Istituto per la Protezione Sostenibile delle Piante, Consiglio Nazionale delle Ricerche, via Amendola 165/A, 70126 Bari, Italy.
Dipartimento di Scienze del Suolo, della Pianta e degli Alimenti, Università degli Studi di Bari Aldo Moro, via Amendola 165/A, 70126 Bari, Italy.
Insects. 2020 Mar 3;11(3):161. doi: 10.3390/insects11030161.
We report the first occurrence of the orange spiny whitefly (; OSW) on the tree of heaven () in Bari, Apulia region, Italy. After our first observation in 2016, the infestation recurred regularly during the following years and expanded to the neighboring trees. Since then, we have also found the insect on numerous patches of the tree of heaven and other plant species in the Bari province. Nevertheless, the tree of heaven was not particularly threatened by the insect, so that a possible contribution by OSW for the control of such an invasive plant cannot be hypothesized hitherto. This work was also aimed at profiling the microbiome of OSW feeding on . For this purpose, we used the denaturing gradient gel electrophoresis (DGGE) and the deep sequencing of small RNAs (sRNAs). Both techniques unveiled the presence of " Portiera" (primary endosymbiont), sp. and sp., endosymbionts already reported for other Aleyrodidae. Deep sequencing data were analyzed by four computational pipelines in order to understand the reliability of the detection of fungi, bacteria, and viruses: Kraken, Kaiju, Velvet, and VelvetOptimiser. Some contigs assembled by Velvet or VelvetOptimiser were associated with insects, but not necessarily in the genus or Aleyrodidae family, suggesting the non-specificity of sRNAs or possible traces of parasitoids in the sample (e.g., sp.). Finally, deep sequencing data were used to describe the microtranscriptome of OSW: 56 canonical and at least four high-confidence novel microRNAs (miRNAs) were identified. The overall miRNA abundance in OSW was in agreement with previous works on , and bantam-3p, miR-276a-3p, miR-317-3p, miR-750-3p, and mir-8-3p were the most represented miRNAs.
我们报告了橙色刺粉虱（学名：Aleurocanthus woglumi；简称：OSW）在意大利普利亚大区巴里市的臭椿（学名：Ailanthus altissima）上首次出现的情况。自2016年我们首次观察到该虫害以来，在随后几年中其反复出现，并蔓延至邻近树木。从那时起，我们还在巴里省的众多臭椿斑块以及其他植物物种上发现了这种昆虫。然而，臭椿并未受到该昆虫的特别威胁，因此，目前还无法推测橙色刺粉虱对控制这种入侵植物可能做出的贡献。这项工作还旨在分析以臭椿为食的橙色刺粉虱的微生物组。为此，我们使用了变性梯度凝胶电泳（DGGE）和小RNA（sRNA）深度测序技术。这两种技术都揭示了“Portiera”（初级内共生菌）、Candidatus Hamiltonella sp.和Candidatus Cardinium sp.的存在，这些内共生菌已在其他粉虱科中被报道过。为了了解真菌、细菌和病毒检测的可靠性，我们通过四个计算流程对深度测序数据进行了分析：Kraken、Kaiju、Velvet和VelvetOptimiser。由Velvet或VelvetOptimiser组装的一些重叠群与昆虫有关，但不一定属于Aleurocanthus属或粉虱科，这表明样本中的sRNA具有非特异性，或者可能存在寄生蜂的痕迹（例如，Encarsia sp.）。最后，深度测序数据被用于描述橙色刺粉虱的微转录组：共鉴定出56个经典的和至少4个高可信度的新型微小RNA（miRNA）。橙色刺粉虱中miRNA的总体丰度与之前对其他粉虱的研究结果一致，其中bantam - 3p、miR - 276a - 3p、miR - 317 - 3p、miR - 750 - 3p和mir - 8 - 3p是最具代表性的miRNA。
