Spasojevic Slobodan D, Stojanovic Vesna D, Barisic Nenad A, Doronjski Aleksandra R, Zikic Dragan R, Babovic Sinisa M
Institute of Child and Youth Healthcare of Vojvodina , Novi Sad , Serbia .
J Matern Fetal Neonatal Med. 2013 Oct;26(15):1506-9. doi: 10.3109/14767058.2013.789846. Epub 2013 May 2.
Evaluation of neuroprotective effects of hypothermia, erythropoietin and their simultaneous use after perinatal asphyxia in newborn rats.
Hysterectomy was performed to Wistar female rats on the last day of gestation. Perinatal asphyxia was induced by submersion of uterus containing pups in saline for 15 min. After resuscitation, pups were randomized into 4 groups, 15 animals in each: G1 - asphyxia; G2 - asphyxia + hypothermia (rectal temperature 33 °C for 1 h); G3 - asphyxia + erythropoietin (Darbepoetin-α 2.5 μg, intraperitoneally) and G4 - asphyxia + erythropoietin + hypothermia. Pups were sacrificed on 7th day of life and histopathological analysis of hippocampus was performed.
Measure of damage to dorsal, ventral and entire hippocampus was significantly lower in groups G2, G3 and G4 than in group G1 (p ~ 0.00; respectively). Measure of damage to hippocampus in group G4 was significantly lower than in group G2 (p = 0.029).
This study demonstrates that simultaneous use of hypothermia and erythropoietin has more expressed neuroprotective effects than sole use of hypothermia after perinatal asphyxia in newborn rats.
评估低温、促红细胞生成素及其联合应用对新生大鼠围产期窒息后的神经保护作用。
在妊娠最后一天对Wistar雌性大鼠进行子宫切除术。将含有幼崽的子宫浸入盐水中15分钟以诱导围产期窒息。复苏后，将幼崽随机分为4组，每组15只动物：G1-窒息组；G2-窒息+低温组（直肠温度33°C，持续1小时）；G3-窒息+促红细胞生成素组（皮下注射达贝泊汀-α 2.5μg）；G4-窒息+促红细胞生成素+低温组。在出生后第7天处死幼崽，并对海马进行组织病理学分析。
G2、G3和G4组背侧、腹侧及整个海马的损伤程度均显著低于G1组（p均约为0.00）。G4组海马损伤程度显著低于G2组（p=0.029）。
本研究表明，在新生大鼠围产期窒息后，低温和促红细胞生成素联合应用比单独使用低温具有更明显的神经保护作用。
