氧化型和还原型细胞色素c及细胞色素f单分子膜的表面性质

Surface properties of monomolecular films of oxidized and reduced cytochrome c and f.

作者信息

Chin P, Brody S S

出版信息

Biochemistry. 1975 Mar 25;14(6):1190-3. doi: 10.1021/bi00677a014.

Abstract

The surface properties of monomolecular films of oxidized and reduced cytochromes f and c were measured at an air-water interface. Area/molecular (A) and surface potential (deltaV) for oxidized and reduced forms of the cytochromes were measured as a function of pH. Oxidized cyt f has a maximum for both A and deltaV at pH 7.5. At a surface pressure of 6 dyn/cm the maximum A equals 2600 plus or minus 50 A2 and the maximum deltaV equals 200 plus or minus 10 mV. Reduced cyt f as a function of pH has a minimum value for both A (2200 A2) and deltaV (95 mV). Oxidized cyt c as a function of pH has minima for A (140 A2) and deltaV (188 mV) at pH 7.0 and 7.3, respectively. On the other hand, reduced cyt has maximum values for A (220 A2) and deltaV (260 mV) at pH 7.0 and 7.3, respectively.

摘要

在空气-水界面测量了氧化型和还原型细胞色素f和c单分子膜的表面性质。测定了细胞色素氧化型和还原型的面积/分子（A）和表面电位（δV）随pH的变化。氧化型细胞色素f在pH 7.5时A和δV均达到最大值。在表面压力为6达因/厘米时，最大A等于2600±50 Å²，最大δV等于200±10毫伏。还原型细胞色素f随pH变化，A（2200 Å²）和δV（95毫伏）均有最小值。氧化型细胞色素c随pH变化，在pH 7.0和7.3时，A（140 Å²）和δV（188毫伏）分别达到最小值。另一方面，还原型细胞色素c在pH 7.0和7.3时，A（220 Å²）和δV（260毫伏）分别达到最大值。