氧化型和还原型细胞色素c在羧甲基纤维素上的色谱分析。
Chromatography of oxidized and reduced cytochrome c on carboxymethylcellulose.
作者信息
Dixon H B, Thompson C M
出版信息
Biochem J. 1968 Apr;107(3):427-31. doi: 10.1042/bj1070427.
Abstract
- Cytochrome c was isolated from horse heart by a chromatographic method. 2. Oxidized and reduced cytochrome c were chromatographed on CM-cellulose that was in equilibrium with several buffer systems of constant composition at pH values of 8.4, 6.75 and 4.9. 3. Separation was better at the higher pH values; the oxidized form was retarded more than twice as much as the reduced form, though they differed by only a single charge. 4. Self-competition between cytochrome molecules is suggested to account for the peak distortion observed at high loads (above 20mum protein concentration).
摘要
- 采用色谱法从马心中分离出细胞色素c。2. 将氧化型和还原型细胞色素c在CM - 纤维素上进行色谱分析，该CM - 纤维素与几种组成恒定的缓冲系统在pH值8.4、6.75和4.9下达到平衡。3. 在较高pH值下分离效果更好；氧化型的滞留时间比还原型多两倍以上，尽管它们仅相差一个电荷。4. 细胞色素分子之间的自竞争被认为是高负载（蛋白质浓度高于20μm）时观察到的峰变形的原因。
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