Department of Orthopaedic Surgery, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts, USA.
J Arthroplasty. 2013 Jun;28(6):918-21. doi: 10.1016/j.arth.2013.02.018. Epub 2013 Mar 23.
A recent study has suggested that irrigation with povidone-iodine solution after knee arthroplasty significantly decreases rates of post-operative surgical site infection. However, there is only limited knowledge of potential chondrotoxic effects on the residual cartilage in patients with partial knee arthroplasties or unresurfaced patella in total knee arthroplasty. Macroscopically normal bovine cartilage explants (n=42) were exposed to different povidone-iodine concentrations for 1, 3 or 6 min, as well as saline control. The viability of superficial chondrocytes was measured by a Live/Dead cytotoxicity assay. Chondrotoxicity correlated positively with the length of exposure, regardless of the concentration. The extent of superficial chondrocyte death was significantly greater at higher concentrations of povidone-iodine solutions. 0.35% povidone-iodine solution was the least chondrotoxic of all concentrations, but still reduced cell viability significantly if applied for longer than 1 min. Our data suggest that povidone-iodine solution at all tested concentrations has a pronounced chondrotoxic effect on the superficial cartilage layer when used for time periods longer than 1 min.
最近的一项研究表明，膝关节置换术后使用聚维酮碘溶液冲洗显著降低了术后手术部位感染的发生率。然而，对于部分膝关节置换术或全膝关节置换术中未覆盖髌骨的患者，其对残留软骨的潜在软骨毒性作用知之甚少。将宏观正常的牛软骨外植体（n=42）暴露于不同浓度的聚维酮碘中 1、3 或 6 分钟，以及生理盐水对照。通过 Live/Dead 细胞毒性测定法测量浅层软骨细胞的活力。无论浓度如何，软骨毒性与暴露时间的长短呈正相关。在较高浓度的聚维酮碘溶液中，浅层软骨细胞死亡的程度明显更大。所有浓度中，0.35%的聚维酮碘溶液的软骨毒性最小，但如果应用时间超过 1 分钟，仍会显著降低细胞活力。我们的数据表明，当使用时间超过 1 分钟时，所有测试浓度的聚维酮碘溶液对浅层软骨层都有明显的软骨毒性作用。
