Romano Veronica, Di Gennaro Donato, Sacco Anna Maria, Festa Enrico, Roscetto Emanuela, Basso Morena Anna, Ascione Tiziana, Balato Giovanni
Section of Human Anatomy, Department of Public Health, "Federico II" University, 80131 Naples, Italy.
Section of Orthopaedic Surgery, Department of Public Health, "Federico II" University, 80131 Naples, Italy.
Diagnostics (Basel). 2022 Aug 21;12(8):2021. doi: 10.3390/diagnostics12082021.
The increasing incidence of periprosthetic joint infections (PJIs) has led to a growing interest in developing strategies to prevent and treat this severe complication. The surgical site's application of antiseptic solutions to eliminate contaminating bacteria and eradicate the bacterial biofilm has been increasing over time. Even though it has been proven that combining antimicrobials could enhance their activities and help overcome acquired microbial resistance related to the topical use of antibiotics, the toxicity of integrated solutions is not well described. This study aimed to evaluate the cytotoxicity of solutions containing povidone-iodine (PI) and hydrogen peroxide (H2O2), alone or in combination, after 1.3 and 5 min of exposure. Chondrocytes, tenocytes, and fibroblast-like synoviocytes were used for cytotoxicity analysis. Trypan blue stain (0.4% in PBS) was applied to evaluate the dead cells. All solutions tested showed a progressive increase in toxicity as exposure time increased except for PI at 0.3%, which exhibited the lowest toxicity. The combined solutions reported a reduced cellular killing at 3 and 5 min than H2O2 at equal concentrations, similar results to PI solutions.
人工关节周围感染（PJI）发病率的不断上升，引发了人们对制定预防和治疗这一严重并发症策略的日益浓厚兴趣。随着时间的推移，在手术部位应用抗菌溶液以消除污染细菌和根除细菌生物膜的情况越来越多。尽管已证明联合使用抗菌药物可增强其活性，并有助于克服与局部使用抗生素相关的获得性微生物耐药性，但复合溶液的毒性尚无详尽描述。本研究旨在评估含聚维酮碘（PI）和过氧化氢（H2O2）的溶液单独或联合使用1.3分钟和5分钟后的细胞毒性。采用软骨细胞、肌腱细胞和成纤维样滑膜细胞进行细胞毒性分析。应用台盼蓝染色（PBS中0.4%）评估死细胞。除0.3%的PI表现出最低毒性外，所有测试溶液的毒性均随暴露时间的增加而逐渐增强。与等浓度的H2O2相比，复合溶液在3分钟和5分钟时的细胞杀伤作用减弱，这与PI溶液的结果相似。
